{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:18:59Z","timestamp":1782350339248,"version":"3.54.5"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: For heterogeneous tissues, measurements of gene expression through mRNA-Seq data are confounded by relative proportions of cell types involved. In this note, we introduce an efficient pipeline: DeconRNASeq, an R package for deconvolution of heterogeneous tissues based on mRNA-Seq data. It adopts a globally optimized non-negative decomposition algorithm through quadratic programming for estimating the mixing proportions of distinctive tissue types in next-generation sequencing data. We demonstrated the feasibility and validity of DeconRNASeq across a range of mixing levels and sources using mRNA-Seq data mixed in silico at known concentrations. We validated our computational approach for various benchmark data, with high correlation between our predicted cell proportions and the real fractions of tissues. Our study provides a rigorous, quantitative and high-resolution tool as a prerequisite to use mRNA-Seq data. The modularity of package design allows an easy deployment of custom analytical pipelines for data from other high-throughput platforms.<\/jats:p>\n               <jats:p>Availability: DeconRNASeq is written in R, and is freely available at http:\/\/bioconductor.org\/packages.<\/jats:p>\n               <jats:p>Contact: \u00a0tinggong@gmail.com<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt090","type":"journal-article","created":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T01:50:54Z","timestamp":1361497854000},"page":"1083-1085","source":"Crossref","is-referenced-by-count":292,"title":["DeconRNASeq: a statistical framework for deconvolution of heterogeneous tissue samples based on mRNA-Seq data"],"prefix":"10.1093","volume":"29","author":[{"given":"Ting","family":"Gong","sequence":"first","affiliation":[{"name":"Biomarker Development, Translational Medicine, Novartis Institutes for BioMedical Research, Cambridge, MA 02139, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph D.","family":"Szustakowski","sequence":"additional","affiliation":[{"name":"Biomarker Development, Translational Medicine, Novartis Institutes for BioMedical Research, Cambridge, MA 02139, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,2,21]]},"reference":[{"key":"2023012810290575400_btt090-B1","volume-title":"Nonlinear Programming","author":"Bertsekas","year":"1999","edition":"2nd"},{"key":"2023012810290575400_btt090-B2","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1093\/bioinformatics\/btr446","article-title":"ContEst: estimating cross-contamination of human samples in next-generation sequencing data","volume":"27","author":"Cibulskis","year":"2011","journal-title":"Bioinformatics"},{"key":"2023012810290575400_btt090-B3","doi-asserted-by":"crossref","first-page":"e27156","DOI":"10.1371\/journal.pone.0027156","article-title":"Optimal deconvolution of transcriptional profiling data using quadratic programming with application to complex clinical blood samples","volume":"6","author":"Gong","year":"2011","journal-title":"PLoS One"},{"key":"2023012810290575400_btt090-B4","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/nbt0510-421","article-title":"Advancing RNA-Seq analysis","volume":"28","author":"Haas","year":"2010","journal-title":"Nat. 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