{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T18:08:36Z","timestamp":1781806116020,"version":"3.54.5"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Cell division in Escherichia coli is morphologically symmetric. However, as unwanted protein aggregates are segregated to the cell poles and, after divisions, accumulate at older poles, generate asymmetries in sister cells\u2019 vitality. Novel single-molecule detection techniques allow observing aging-related processes in vivo, over multiple generations, informing on the underlying mechanisms.<\/jats:p>\n               <jats:p>Results: CellAging is a tool to automatically extract information on polar segregation and partitioning in division of aggregates in E.coli, and on cellular vitality. From time-lapse, parallel brightfield and fluorescence microscopy images, it performs cell segmentation, alignment of brightfield and fluorescence images, lineage construction and pole age determination, and it computes aging-related features. We exemplify its use by analyzing spatial distributions of fluorescent protein aggregates from images of cells across generations.<\/jats:p>\n               <jats:p>Availability: CellAging, instructions and an example are available at http:\/\/www.cs.tut.fi\/%7esanchesr\/cellaging\/.<\/jats:p>\n               <jats:p>Contact: \u00a0andre.ribeiro@tut.fi<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt194","type":"journal-article","created":{"date-parts":[[2013,4,24]],"date-time":"2013-04-24T03:35:45Z","timestamp":1366774545000},"page":"1708-1709","source":"Crossref","is-referenced-by-count":33,"title":["CellAging: a tool to study segregation and partitioning in division in cell lineages of <i>Escherichia coli<\/i>"],"prefix":"10.1093","volume":"29","author":[{"given":"Antti","family":"H\u00e4kkinen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anantha-Barathi","family":"Muthukrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Mora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose M.","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre S.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,4,23]]},"reference":[{"key":"2023062614294474200_btt194-B1","doi-asserted-by":"crossref","first-page":"R100","DOI":"10.1186\/gb-2006-7-10-r100","article-title":"Cellprofiler: image analysis software for identifying and quantifying cell phenotypes","volume":"7","author":"Carpenter","year":"2006","journal-title":"Genome Biol."},{"key":"2023062614294474200_btt194-B2","volume-title":"Classification and Regression Trees","author":"Breiman","year":"1984"},{"key":"2023062614294474200_btt194-B3","doi-asserted-by":"crossref","first-page":"3076","DOI":"10.1073\/pnas.0708931105","article-title":"Asymmetric segregation of protein aggregates is associated with cellular aging and rejuvenation","volume":"105","author":"Lindner","year":"2008","journal-title":"Proc. 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