{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:43:02Z","timestamp":1767962582458,"version":"3.49.0"},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"15","license":[{"start":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:00:00Z","timestamp":1475366400000},"content-version":"vor","delay-in-days":1224,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: We present Mutascope, a sequencing analysis pipeline specifically developed for the identification of somatic variants present at low-allelic fraction from high-throughput sequencing of amplicons from matched tumor-normal specimen. Using datasets reproducing tumor genetic heterogeneity, we demonstrate that Mutascope has a higher sensitivity and generates fewer false-positive calls than tools designed for shotgun sequencing or diploid genomes.<\/jats:p>\n               <jats:p>Availability: Freely available on the web at http:\/\/sourceforge.net\/projects\/mutascope\/.<\/jats:p>\n               <jats:p>Contact: \u00a0oharismendy@ucsd.edu<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt305","type":"journal-article","created":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T00:49:43Z","timestamp":1369702183000},"page":"1908-1909","source":"Crossref","is-referenced-by-count":19,"title":["Mutascope: sensitive detection of somatic mutations from deep amplicon sequencing"],"prefix":"10.1093","volume":"29","author":[{"given":"Shawn E.","family":"Yost","sequence":"first","affiliation":[{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"}]},{"given":"Hakan","family":"Alakus","sequence":"additional","affiliation":[{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"}]},{"given":"Hiroko","family":"Matsui","sequence":"additional","affiliation":[{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"}]},{"given":"Richard B.","family":"Schwab","sequence":"additional","affiliation":[{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"}]},{"given":"Kristen","family":"Jepsen","sequence":"additional","affiliation":[{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"}]},{"given":"Kelly A.","family":"Frazer","sequence":"additional","affiliation":[{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"}]},{"given":"Olivier","family":"Harismendy","sequence":"additional","affiliation":[{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and Translational Research Institute and 7Institute for Genomic Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA"},{"name":"1 Bioinformatics Graduate Program, 2Department of Pediatrics, 3Rady Children\u2019s Hospital, 4Moores UCSD Cancer Center, 5Department of Medicine, 6Clinical and 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