{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T02:53:53Z","timestamp":1785984833123,"version":"3.56.0"},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: An ultrafast DNA sequence aligner (Isaac Genome Alignment Software) that takes advantage of high-memory hardware (&amp;gt;48 GB) and variant caller (Isaac Variant Caller) have been developed. We demonstrate that our combined pipeline (Isaac) is four to five times faster than BWA + GATK on equivalent hardware, with comparable accuracy as measured by trio conflict rates and sensitivity. We further show that Isaac is effective in the detection of disease-causing variants and can easily\/economically be run on commodity hardware.<\/jats:p>\n               <jats:p>Availability: Isaac has an open source license and can be obtained at https:\/\/github.com\/sequencing.<\/jats:p>\n               <jats:p>Contact: \u00a0craczy@illumina.com<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt314","type":"journal-article","created":{"date-parts":[[2013,6,5]],"date-time":"2013-06-05T02:31:51Z","timestamp":1370399511000},"page":"2041-2043","source":"Crossref","is-referenced-by-count":312,"title":["Isaac: ultra-fast whole-genome secondary analysis on Illumina sequencing platforms"],"prefix":"10.1093","volume":"29","author":[{"given":"Come","family":"Raczy","sequence":"first","affiliation":[{"name":"1 Illumina United Kingdom, Chesterford Research Park, Little 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