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SAMstrt is an extension code for SAMseq, which is a statistical method for differential expression, to enable spike-in normalization and statistical testing based on the estimated absolute number of transcripts per cell for single-cell RNA-seq methods.<\/jats:p>\n               <jats:p>Availability and Implementation: SAMstrt is implemented on R and available in github (https:\/\/github.com\/shka\/R-SAMstrt).<\/jats:p>\n               <jats:p>Contact: \u00a0shintaro.katayama@ki.se<\/jats:p>\n               <jats:p>Supplementary Information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt511","type":"journal-article","created":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:34:29Z","timestamp":1377995669000},"page":"2943-2945","source":"Crossref","is-referenced-by-count":108,"title":["SAMstrt: statistical test for differential expression in single-cell transcriptome with spike-in normalization"],"prefix":"10.1093","volume":"29","author":[{"given":"Shintaro","family":"Katayama","sequence":"first","affiliation":[{"name":"1 Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden, 2Science for Life Laboratory, Karolinska Institutet Science Park, 171 21 Solna, Sweden and 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden"},{"name":"1 Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden, 2Science for Life Laboratory, Karolinska Institutet Science Park, 171 21 Solna, Sweden and 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virpi","family":"T\u00f6h\u00f6nen","sequence":"additional","affiliation":[{"name":"1 Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden, 2Science for Life Laboratory, Karolinska Institutet Science Park, 171 21 Solna, Sweden and 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sten","family":"Linnarsson","sequence":"additional","affiliation":[{"name":"1 Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden, 2Science for Life Laboratory, Karolinska Institutet Science Park, 171 21 Solna, Sweden and 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juha","family":"Kere","sequence":"additional","affiliation":[{"name":"1 Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden, 2Science for Life Laboratory, Karolinska Institutet Science Park, 171 21 Solna, Sweden and 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden"},{"name":"1 Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden, 2Science for Life Laboratory, Karolinska Institutet Science Park, 171 21 Solna, Sweden and 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,8,31]]},"reference":[{"key":"2023012810485346600_btt511-B1","doi-asserted-by":"crossref","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","article-title":"Differential expression analysis for sequence count data","volume":"11","author":"Anders","year":"2010","journal-title":"Genome Biol."},{"key":"2023012810485346600_btt511-B2","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/1471-2105-11-94","article-title":"Evaluation of statistical methods for normalization and differential expression in mRNA-Seq experiments","volume":"11","author":"Bullard","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"2023012810485346600_btt511-B3","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1016\/j.cub.2006.03.092","article-title":"Transcriptional pulsing of a developmental gene","volume":"16","author":"Chubb","year":"2006","journal-title":"Curr. 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