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Access to &amp;gt;28.5 million sequences and 300 million features is provided through a well-documented and flexible RESTful interface that supports fetching specific data subsets, bulk queries, visualization and searching by MD5 digests or external database identifiers. We have also developed an HTML5\/JavaScript web application exemplifying how to interact with SeqDepot and Perl\/Python scripts for use with local processing pipelines.<\/jats:p>\n               <jats:p>Availability: Freely available on the web at http:\/\/seqdepot.net\/. REST access via http:\/\/seqdepot.net\/api\/v1. Database files and scripts may be downloaded from http:\/\/seqdepot.net\/download.<\/jats:p>\n               <jats:p>Contact: \u00a0ulrich.luke+sci@gmail.com<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt658","type":"journal-article","created":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T01:30:49Z","timestamp":1384479049000},"page":"295-297","source":"Crossref","is-referenced-by-count":10,"title":["SeqDepot: streamlined database of biological sequences and precomputed features"],"prefix":"10.1093","volume":"30","author":[{"given":"Luke E.","family":"Ulrich","sequence":"first","affiliation":[{"name":"1 Agile Genomics, LLC, Mount Pleasant, SC 29466, USA, 2Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA and 3Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA"},{"name":"1 Agile Genomics, LLC, Mount Pleasant, SC 29466, USA, 2Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA and 3Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor B.","family":"Zhulin","sequence":"additional","affiliation":[{"name":"1 Agile Genomics, LLC, Mount Pleasant, SC 29466, USA, 2Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA and 3Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA"},{"name":"1 Agile Genomics, LLC, Mount Pleasant, SC 29466, USA, 2Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA and 3Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,11,13]]},"reference":[{"key":"2023012710392403500_btt658-B1","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"2023012710392403500_btt658-B2","doi-asserted-by":"crossref","first-page":"bas019","DOI":"10.1093\/database\/bas019","article-title":"The PRINTS database: a fine-grained protein sequence annotation and analysis resource\u2014its status in 2012","volume":"2012","author":"Attwood","year":"2012","journal-title":"Database"},{"key":"2023012710392403500_btt658-B3","doi-asserted-by":"crossref","first-page":"D71","DOI":"10.1093\/nar\/gkr981","article-title":"Reorganizing the protein space at the Universal Protein Resource (UniProt)","volume":"40","author":"Consortium","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2023012710392403500_btt658-B4","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/bioinformatics\/btg394","article-title":"TM or not TM: transmembrane protein prediction with low false positive rate using DAS-TMfilter","volume":"20","author":"Cserzo","year":"2003","journal-title":"Bioinformatics"},{"key":"2023012710392403500_btt658-B5","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1038\/nprot.2007.131","article-title":"Locating proteins in the cell using TargetP, SignalP and related tools","volume":"2","author":"Emanuelsson","year":"2007","journal-title":"Nat. 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