{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:25:38Z","timestamp":1779243938740,"version":"3.51.4"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Microtubules are dynamic polymers of tubulin dimers that undergo continuous assembly and disassembly. A mounting number of microtubule-associated proteins (MAPs) regulate the dynamic behavior of microtubules and hence the assembly and disassembly of disparate microtubule structures within the cell. Despite recent advances in identification and functional characterization of MAPs, a substantial number of microtubule accessory factors have not been functionally annotated. Here, using profile-to-profile comparisons and structure modeling, we show that the yeast outer kinetochore components NDC80 and NUF2 share evolutionary ancestry with a novel protein family in mammals comprising, besides NDC80\/HEC1 and NUF2, three Intraflagellar Transport (IFT) complex B subunits (IFT81, IFT57, CLUAP1) as well as six proteins with poorly defined function (FAM98A-C, CCDC22, CCDC93 and C14orf166). We show that these proteins consist of a divergent N-terminal calponin homology (CH)-like domain adjoined to an array of C-terminal heptad repeats predicted to form a coiled-coil arrangement. We have named the divergent CH-like domain NN\u2013CH after the founding members NDC80 and NUF2.<\/jats:p>\n               <jats:p>Contact: kbschou@bio.ku.dk or lbpedersen@bio.ku.dk<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt661","type":"journal-article","created":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T01:25:33Z","timestamp":1384997133000},"page":"899-902","source":"Crossref","is-referenced-by-count":39,"title":["A divergent calponin homology (NN\u2013CH) domain defines a novel family: implications for evolution of ciliary IFT complex B proteins"],"prefix":"10.1093","volume":"30","author":[{"given":"Kenneth B.","family":"Schou","sequence":"first","affiliation":[{"name":"1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark and 2Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen OE, Denmark"},{"name":"1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark and 2Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen OE, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens S.","family":"Andersen","sequence":"additional","affiliation":[{"name":"1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark and 2Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen OE, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lotte B.","family":"Pedersen","sequence":"additional","affiliation":[{"name":"1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark and 2Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen OE, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,11,20]]},"reference":[{"key":"2023012710453232800_btt661-B1","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1038\/nature02166","article-title":"Proteomic characterization of the human centrosome by protein correlation profiling","volume":"426","author":"Andersen","year":"2003","journal-title":"Nature"},{"key":"2023012710453232800_btt661-B2","doi-asserted-by":"crossref","first-page":"bap022","DOI":"10.1093\/database\/bap022","article-title":"Cildb: a knowledgebase for centrosomes and cilia","volume":"2009","author":"Arnaiz","year":"2009","journal-title":"Database (Oxford)"},{"key":"2023012710453232800_btt661-B3","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1126\/science.1240985","article-title":"Molecular basis of tubulin transport within the cilium by IFT74 and IFT81","volume":"341","author":"Bhogaraju","year":"2013","journal-title":"Science"},{"key":"2023012710453232800_btt661-B4","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1073\/pnas.0810767106","article-title":"Sequence context-specific profiles for homology searching","volume":"106","author":"Biegert","year":"2009","journal-title":"Proc. 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