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CasOT is a local tool designed to find potential off-target sites in any given genome or user-provided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches allowed in the seed and non-seed regions.<\/jats:p>\n               <jats:p>Availability: \u00a0http:\/\/eendb.zfgenetics.org\/casot\/<\/jats:p>\n               <jats:p>Contact: \u00a0zfgenetics@gmail.com or bzhang@pku.edu.cn<\/jats:p>\n               <jats:p>Supplementary Information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt764","type":"journal-article","created":{"date-parts":[[2014,1,4]],"date-time":"2014-01-04T02:03:14Z","timestamp":1388800994000},"page":"1180-1182","source":"Crossref","is-referenced-by-count":340,"title":["CasOT: a genome-wide Cas9\/gRNA off-target searching tool"],"prefix":"10.1093","volume":"30","author":[{"given":"An","family":"Xiao","sequence":"first","affiliation":[{"name":"1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, 2State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China and 3Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}]},{"given":"Zhenchao","family":"Cheng","sequence":"additional","affiliation":[{"name":"1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, 2State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China and 3Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}]},{"given":"Lei","family":"Kong","sequence":"additional","affiliation":[{"name":"1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, 2State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China and 3Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}]},{"given":"Zuoyan","family":"Zhu","sequence":"additional","affiliation":[{"name":"1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, 2State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China and 3Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}]},{"given":"Shuo","family":"Lin","sequence":"additional","affiliation":[{"name":"1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, 2State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China and 3Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}]},{"given":"Ge","family":"Gao","sequence":"additional","affiliation":[{"name":"1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, 2State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China and 3Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, 2State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China and 3Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA"}]}],"member":"286","published-online":{"date-parts":[[2014,1,2]]},"reference":[{"key":"2023012710490426100_btt764-B1","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"2023012710490426100_btt764-B2","doi-asserted-by":"crossref","first-page":"9584","DOI":"10.1093\/nar\/gkt714","article-title":"CRISPR\/Cas9 systems targeting beta-globin and CCR5 genes have substantial off-target activity","volume":"41","author":"Cradick","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2023012710490426100_btt764-B3","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1038\/nbt.2623","article-title":"High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells","volume":"31","author":"Fu","year":"2013","journal-title":"Nat. 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