{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T08:39:29Z","timestamp":1786437569179,"version":"3.56.0"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T00:00:00Z","timestamp":1478131200000},"content-version":"vor","delay-in-days":770,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Motivation: A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard workflows, custom scripts are needed.<\/jats:p>\n                  <jats:p>Results: We present HTSeq, a Python library to facilitate the rapid development of such scripts. HTSeq offers parsers for many common data formats in HTS projects, as well as classes to represent data, such as genomic coordinates, sequences, sequencing reads, alignments, gene model information and variant calls, and provides data structures that allow for querying via genomic coordinates. We also present htseq-count, a tool developed with HTSeq that preprocesses RNA-Seq data for differential expression analysis by counting the overlap of reads with genes.<\/jats:p>\n                  <jats:p>Availability and implementation: HTSeq is released as an open-source software under the GNU General Public Licence and available from http:\/\/www-huber.embl.de\/HTSeq or from the Python Package Index at https:\/\/pypi.python.org\/pypi\/HTSeq .<\/jats:p>\n                  <jats:p>Contact: \u00a0sanders@fs.tum.de<\/jats:p>","DOI":"10.1093\/bioinformatics\/btu638","type":"journal-article","created":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T23:04:09Z","timestamp":1411772649000},"page":"166-169","source":"Crossref","is-referenced-by-count":19571,"title":["HTSeq\u2014a Python framework to work with high-throughput sequencing data"],"prefix":"10.1093","volume":"31","author":[{"given":"Simon","family":"Anders","sequence":"first","affiliation":[{"name":"Genome Biology Unit, European Molecular Biology Laboratory, 69111 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul Theodor","family":"Pyl","sequence":"additional","affiliation":[{"name":"Genome Biology Unit, European Molecular Biology Laboratory, 69111 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Huber","sequence":"additional","affiliation":[{"name":"Genome Biology Unit, European Molecular Biology Laboratory, 69111 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,9,25]]},"reference":[{"key":"2023020116140904100_btu638-B1","first-page":"129","article-title":"SWIG: an easy to use tool for integrating scripting languages with C and C++","volume-title":"Proceedings of the 4th USENIX Tcl\/Tk workshop","author":"Beazley","year":"1996"},{"key":"2023020116140904100_btu638-B2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1109\/MCSE.2010.118","article-title":"Cython: the best of both worlds","volume":"13","author":"Behnel","year":"2011","journal-title":"Comput. 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