{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T12:13:09Z","timestamp":1781871189553,"version":"3.54.5"},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: \u00a0NucleusJ is a simple and user-friendly ImageJ plugin dedicated to the characterization of nuclear morphology and chromatin organization in 3D. Starting from image stacks, the nuclear boundary is delimited by combining the Otsu segmentation method with optimization of nuclear sphericity. Chromatin domains are segmented by partitioning the nucleus using a 3D watershed algorithm and by thresholding a contrast measure over the resulting regions. As output, NucleusJ quantifies 15 parameters including shape and size of nuclei as well as intra-nuclear objects and their position within the nucleus. A step-by-step documentation is available for self-training, together with data sets of nuclei with different nuclear organization.<\/jats:p>\n               <jats:p>Availability and implementation: Dataset of nuclei is available at https:\/\/www.gred-clermont.fr\/media\/WorkDirectory.zip. NucleusJ is available at http:\/\/imagejdocu.tudor.lu\/doku.php?id=plugin:stacks:nuclear_analysis_plugin:start.<\/jats:p>\n               <jats:p>Contact: \u00a0philippe.andrey@versailles.inra.fr and christophe.tatout@univ-bpclermont.fr<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btu774","type":"journal-article","created":{"date-parts":[[2014,11,22]],"date-time":"2014-11-22T02:58:16Z","timestamp":1416625096000},"page":"1144-1146","source":"Crossref","is-referenced-by-count":54,"title":["<i>NucleusJ<\/i>: an ImageJ plugin for quantifying 3D images of interphase nuclei"],"prefix":"10.1093","volume":"31","author":[{"given":"Axel","family":"Poulet","sequence":"first","affiliation":[{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Arganda-Carreras","sequence":"additional","affiliation":[{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Legland","sequence":"additional","affiliation":[{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"},{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline V.","family":"Probst","sequence":"additional","affiliation":[{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Andrey","sequence":"additional","affiliation":[{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"},{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Tatout","sequence":"additional","affiliation":[{"name":"1 UMR CNRS 6293 INSERM U 1103, GRED, Clermont Universit\u00e9, Aubi\u00e8re, France, 2UMR INRA-AgroParisTech 1318 ERL CNRS 3559, IJPB, Versailles, France, 3UMR INRA-AgroParisTech 782, Food Process Engineering and Microbiology, Thiverval-Grignon, France and 4Sorbonne Universit\u00e9s, UPMC Univ Paris 06 UFR 927, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,11,20]]},"reference":[{"key":"2023051309142625500_btu774-B1","doi-asserted-by":"crossref","first-page":"e1000853","DOI":"10.1371\/journal.pcbi.1000853","article-title":"Statistical analysis of 3D images detects regular spatial distributions of centromeres and chromocenters in animal and plant nuclei","volume":"6","author":"Andrey","year":"2010","journal-title":"PLoS Comput. 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