{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T18:12:21Z","timestamp":1776795141271,"version":"3.51.2"},"reference-count":6,"publisher":"Oxford University Press (OUP)","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A major roadblock towards accurate interpretation of single cell RNA-seq data is large technical noise resulted from small amount of input materials. The existing methods mainly aim to find differentially expressed genes rather than directly de-noise the single cell data. We present here a powerful but simple method to remove technical noise and explicitly compute the true gene expression levels based on spike-in ERCC molecules.<\/jats:p>\n               <jats:p>Availability and implementation: The software is implemented by R and the download version is available at http:\/\/wanglab.ucsd.edu\/star\/GRM.<\/jats:p>\n               <jats:p>Contact: \u00a0wei-wang@ucsd.edu<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btv122","type":"journal-article","created":{"date-parts":[[2015,2,26]],"date-time":"2015-02-26T15:34:19Z","timestamp":1424964859000},"page":"2225-2227","source":"Crossref","is-referenced-by-count":93,"title":["Normalization and noise reduction for single cell RNA-seq experiments"],"prefix":"10.1093","volume":"31","author":[{"given":"Bo","family":"Ding","sequence":"first","affiliation":[{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Zheng","sequence":"additional","affiliation":[{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Zhu","sequence":"additional","affiliation":[{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyang","family":"Jia","sequence":"additional","affiliation":[{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA"},{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rizi","family":"Ai","sequence":"additional","affiliation":[{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre","family":"Wildberg","sequence":"additional","affiliation":[{"name":"1 Department of Chemistry and Biochemistry, University of California, La Jolla, CA 92093, USA, 2College of Computer Science and Technology, Jilin University, Changchun 130012, China and 3Department of Cellular and 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