{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T21:45:14Z","timestamp":1776116714646,"version":"3.50.1"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"19","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Motivation: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.<\/jats:p>\n                  <jats:p>Results: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.<\/jats:p>\n                  <jats:p>Availability and implementation: Software implemented in Python and datasets available for download from http:\/\/busco.ezlab.org.<\/jats:p>\n                  <jats:p>Contact: \u00a0evgeny.zdobnov@unige.ch<\/jats:p>\n                  <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btv351","type":"journal-article","created":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T00:09:00Z","timestamp":1433981340000},"page":"3210-3212","source":"Crossref","is-referenced-by-count":12353,"title":["BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs"],"prefix":"10.1093","volume":"31","author":[{"given":"Felipe A.","family":"Sim\u00e3o","sequence":"first","affiliation":[{"name":"Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland"}]},{"given":"Robert M.","family":"Waterhouse","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland"}]},{"given":"Panagiotis","family":"Ioannidis","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland"}]},{"given":"Evgenia V.","family":"Kriventseva","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland"}]},{"given":"Evgeny M.","family":"Zdobnov","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland"}]}],"member":"286","published-online":{"date-parts":[[2015,6,9]]},"reference":[{"key":"2023020202302672300_btv351-B1","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1093\/bioinformatics\/bts723","article-title":"ALE: a generic assembly likelihood evaluation framework for assessing the accuracy of genome and metagenome assemblies","volume":"29","author":"Clark","year":"2013","journal-title":"Bioinformatics"},{"key":"2023020202302672300_btv351-B2","doi-asserted-by":"crossref","first-page":"e1002195","DOI":"10.1371\/journal.pcbi.1002195","article-title":"Accelerated profile HMM searches","volume":"7","author":"Eddy","year":"2011","journal-title":"PLoS Comput. 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