{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T13:04:26Z","timestamp":1761311066616},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T00:00:00Z","timestamp":1476230400000},"content-version":"vor","delay-in-days":399,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Read simulators combined with alignment evaluation tools provide the most straightforward way to evaluate and compare mappers. Simulation of reads is accompanied by information about their positions in the source genome. This information is then used to evaluate alignments produced by the mapper. Finally, reports containing statistics of successful read alignments are created.<\/jats:p>\n               <jats:p>In default of standards for encoding read origins, every evaluation tool has to be made explicitly compatible with the simulator used to generate reads.<\/jats:p>\n               <jats:p>Results: To solve this obstacle, we have created a generic format Read Naming Format (Rnf) for assigning read names with encoded information about original positions. Futhermore, we have developed an associated software package RnfTools containing two principal components. MIShmash applies one of popular read simulating tools (among DwgSim, Art, Mason, CuReSim, etc.) and transforms the generated reads into Rnf format. LAVEnder evaluates then a given read mapper using simulated reads in Rnf format. A special attention is payed to mapping qualities that serve for parametrization of Roc curves, and to evaluation of the effect of read sample contamination.<\/jats:p>\n               <jats:p>Availability and implementation: RnfTools: http:\/\/karel-brinda.github.io\/rnftools Spec. of Rnf: http:\/\/karel-brinda.github.io\/rnf-spec<\/jats:p>\n               <jats:p>Contact: \u00a0karel.brinda@univ-mlv.fr<\/jats:p>","DOI":"10.1093\/bioinformatics\/btv524","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T13:37:06Z","timestamp":1441892226000},"page":"136-139","source":"Crossref","is-referenced-by-count":7,"title":["RNF: a general framework to evaluate NGS read mappers"],"prefix":"10.1093","volume":"32","author":[{"given":"Karel","family":"B\u0159inda","sequence":"first","affiliation":[{"name":"1 LIGM\/CNRS, Universit\u00e9 Paris-Est, 77454 Marne-la-Vall\u00e9e, France,"}]},{"given":"Valentina","family":"Boeva","sequence":"additional","affiliation":[{"name":"2 Inserm, U900, Bioinformatics, Biostatistics, Epidemiology and Computational Systems Biology of Cancer, 75248 Paris, France,"},{"name":"3 Institut Curie, Centre de Recherche, 26 rue d\u2019Ulm, 75248 Paris, France and"},{"name":"4 Mines ParisTech, 77300 Fontainebleau, France"}]},{"given":"Gregory","family":"Kucherov","sequence":"additional","affiliation":[{"name":"1 LIGM\/CNRS, Universit\u00e9 Paris-Est, 77454 Marne-la-Vall\u00e9e, France,"}]}],"member":"286","published-online":{"date-parts":[[2015,9,9]]},"reference":[{"key":"2023020110215503900_btv524-B1","doi-asserted-by":"crossref","first-page":"i420","DOI":"10.1093\/bioinformatics\/btq365","article-title":"Characteristics of 454 pyrosequencing data\u2014enabling realistic simulation with flowsim","volume":"26","author":"Balzer","year":"2010","journal-title":"Bioinformatics"},{"key":"2023020110215503900_btv524-B2","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1186\/1471-2164-15-264","article-title":"Comparison of mapping algorithms used in high-throughput sequencing: application to Ion Torrent data","volume":"15","author":"Caboche","year":"2014","journal-title":"BMC Genomics"},{"key":"2023020110215503900_btv524-B3","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1038\/nmeth.2722","article-title":"Systematic evaluation of spliced alignment programs for RNA-seq data","volume":"10","author":"Engstr\u00f6m","year":"2013","journal-title":"Nat. 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