{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T14:41:32Z","timestamp":1759588892527},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: One of the most promising applications of biosynthetic methods is to produce chemical products of high value from the ready-made chemicals. To explore the biosynthetic potentials of a chemical as a synthesis precursor, biosynthetic databases and related chemoinformatics tools are urgently needed. In the present work, a web-based tool, BioSynther, is developed to explore the biosynthetic potentials of precursor chemicals using BKM-react, Rhea, and more than 50\u2009000 in-house RxnFinder reactions manually curated. BioSynther allows researchers to explore biosynthetic potentials, through so far known biochemical reactions, step by step interactively, which could be used as a useful tool in metabolic engineering and synthetic biology.<\/jats:p>\n               <jats:p>Availability and implementation: BioSynther is available at: http:\/\/www.lifemodules.org\/BioSynther\/.<\/jats:p>\n               <jats:p>Contact: hu_qn@tib.cas.cn.<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btv599","type":"journal-article","created":{"date-parts":[[2015,10,16]],"date-time":"2015-10-16T00:48:17Z","timestamp":1444956497000},"page":"472-473","source":"Crossref","is-referenced-by-count":15,"title":["BioSynther: a customized biosynthetic potential explorer"],"prefix":"10.1093","volume":"32","author":[{"given":"Weizhong","family":"Tu","sequence":"first","affiliation":[{"name":"1 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People\u2019s Republic of China,"}]},{"given":"Haoran","family":"Zhang","sequence":"additional","affiliation":[{"name":"2 State Key Laboratory of Software Engineering (Wuhan University) and Wuhan University School of Computer Sciences, Wuhan 430072, People\u2019s Republic of China and"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"2 State Key Laboratory of Software Engineering (Wuhan University) and Wuhan University School of Computer Sciences, Wuhan 430072, People\u2019s Republic of China and"}]},{"given":"Qian-Nan","family":"Hu","sequence":"additional","affiliation":[{"name":"1 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People\u2019s Republic of China,"},{"name":"3 Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, People\u2019s Republic of China"}]}],"member":"286","published-online":{"date-parts":[[2015,10,14]]},"reference":[{"key":"2023020110312908200_btv599-B1","doi-asserted-by":"crossref","first-page":"D754","DOI":"10.1093\/nar\/gkr1126","article-title":"Rhea\u2013a manually curated resource of biochemical reactions","volume":"40","author":"Alcantara","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2023020110312908200_btv599-B2","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/nature06450","article-title":"Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels","volume":"451","author":"Atsumi","year":"2008","journal-title":"Nature"},{"key":"2023020110312908200_btv599-B3","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/1752-0509-5-122","article-title":"A retrosynthetic biology approach to metabolic pathway design for therapeutic production","volume":"5","author":"Carbonell","year":"2011","journal-title":"BMC Syst. 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