{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T19:52:28Z","timestamp":1772308348514,"version":"3.50.1"},"reference-count":4,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T00:00:00Z","timestamp":1476230400000},"content-version":"vor","delay-in-days":286,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The wound healing assay (or scratch assay) is a technique frequently used to quantify the dependence of cell motility\u2014a central process in tissue repair and evolution of disease\u2014subject to various treatments conditions. However processing the resulting data is a laborious task due its high throughput and variability across images. This Robust Quantitative Scratch Assay algorithm introduced statistical outputs where migration rates are estimated, cellular behaviour is distinguished and outliers are identified among groups of unique experimental conditions. Furthermore, the RQSA decreased measurement errors and increased accuracy in the wound boundary at comparable processing times compared to previously developed method (TScratch).<\/jats:p>\n               <jats:p>Availability and implementation: The RQSA is freely available at: http:\/\/ophid.utoronto.ca\/RQSA\/RQSA_Scripts.zip. The image sets used for training and validation and results are available at: (http:\/\/ophid.utoronto.ca\/RQSA\/trainingSet.zip, http:\/\/ophid.utoronto.ca\/RQSA\/validationSet.zip, http:\/\/ophid.utoronto.ca\/RQSA\/ValidationSetResults.zip, http:\/\/ophid.utoronto.ca\/RQSA\/ValidationSet_H1975.zip, http:\/\/ophid.utoronto.ca\/RQSA\/ValidationSet_H1975Results.zip, http:\/\/ophid.utoronto.ca\/RQSA\/RobustnessSet.zip, http:\/\/ophid.utoronto.ca\/RQSA\/RobustnessSet.zip). Supplementary Material is provided for detailed description of the development of the RQSA.<\/jats:p>\n               <jats:p>Contact: \u00a0juris@ai.utoronto.ca<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btv746","type":"journal-article","created":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T02:19:20Z","timestamp":1451614760000},"page":"1439-1440","source":"Crossref","is-referenced-by-count":13,"title":["Robust quantitative scratch assay"],"prefix":"10.1093","volume":"32","author":[{"given":"Andrea","family":"Vargas","sequence":"first","affiliation":[{"name":"1 Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada,"}]},{"given":"Marc","family":"Angeli","sequence":"additional","affiliation":[{"name":"2 Princess Margaret Cancer Centre and Techna, University Health Network, Toronto, ON, Canada,"}]},{"given":"Chiara","family":"Pastrello","sequence":"additional","affiliation":[{"name":"2 Princess Margaret Cancer Centre and Techna, University Health Network, Toronto, ON, Canada,"}]},{"given":"Rosanne","family":"McQuaid","sequence":"additional","affiliation":[{"name":"3 Department of Physiology, University of Toronto, Toronto, ON, Canada,"},{"name":"4 Lunenfeld Tanenbaum Research Institute, Toronto, ON, Canada,"}]},{"given":"Han","family":"Li","sequence":"additional","affiliation":[{"name":"4 Lunenfeld Tanenbaum Research Institute, Toronto, ON, Canada,"}]},{"given":"Andrea","family":"Jurisicova","sequence":"additional","affiliation":[{"name":"3 Department of Physiology, University of Toronto, Toronto, ON, Canada,"},{"name":"4 Lunenfeld Tanenbaum Research Institute, Toronto, ON, Canada,"},{"name":"5 Department of Obstetrics and Gynecology and"}]},{"given":"Igor","family":"Jurisica","sequence":"additional","affiliation":[{"name":"1 Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada,"},{"name":"2 Princess Margaret Cancer Centre and Techna, University Health Network, Toronto, ON, Canada,"},{"name":"6 Department of Computer Science, University of Toronto, Toronto, ON, Canada"}]}],"member":"286","published-online":{"date-parts":[[2015,12,31]]},"reference":[{"key":"2023020112264597800_btv746-B1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.2144\/000113083","article-title":"TScratch: a novel and simple software tool for automated analysis of monolayer wound healing assays","volume":"46","author":"Geb\u00e4ck","year":"2009","journal-title":"Biotechniques"},{"key":"2023020112264597800_btv746-B2","volume-title":"Digital Image Processing Using MATLAB","author":"Gonzalez","year":"2003"},{"key":"2023020112264597800_btv746-B3","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4161\/cam.36224","article-title":"An introduction to the wound healing assay using live-cell microscopy","volume":"8","author":"Jonkman","year":"2014","journal-title":"Cell Adhesion Migration"},{"key":"2023020112264597800_btv746-B4","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1002\/cyto.a.21029","article-title":"A high throughput, interactive imaging, bright-field","volume":"79","author":"Zordan","year":"2011","journal-title":"Cytometry A"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/32\/9\/1439\/49019434\/bioinformatics_32_9_1439.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/32\/9\/1439\/49019434\/bioinformatics_32_9_1439.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T22:30:41Z","timestamp":1675290641000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/32\/9\/1439\/1743750"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,31]]},"references-count":4,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,5,1]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btv746","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2016,5,1]]},"published":{"date-parts":[[2015,12,31]]}}}