{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T06:07:43Z","timestamp":1780553263591,"version":"3.54.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T00:00:00Z","timestamp":1477612800000},"content-version":"vor","delay-in-days":260,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Motivation: Understanding the occurrence and regulation of alternative splicing (AS) is a key task towards explaining the regulatory processes that shape the complex transcriptomes of higher eukaryotes. With the advent of high-throughput sequencing of RNA (RNA-Seq), the diversity of AS transcripts could be measured at an unprecedented depth. Although the catalog of known AS events has grown ever since, novel transcripts are commonly observed when working with less well annotated organisms, in the context of disease, or within large populations. Whereas an identification of complete transcripts is technically challenging and computationally expensive, focusing on single splicing events as a proxy for transcriptome characteristics is fruitful and sufficient for a wide range of analyses.<\/jats:p>\n                  <jats:p>Results: We present SplAdder, an alternative splicing toolbox, that takes RNA-Seq alignments and an annotation file as input to (i) augment the annotation based on RNA-Seq evidence, (ii) identify alternative splicing events present in the augmented annotation graph, (iii) quantify and confirm these events based on the RNA-Seq data and (iv) test for significant quantitative differences between samples. Thereby, our main focus lies on performance, accuracy and usability.<\/jats:p>\n                  <jats:p>Availability: Source code and documentation are available for download at http:\/\/github.com\/ratschlab\/spladder. Example data, introductory information and a small tutorial are accessible via http:\/\/bioweb.me\/spladder.<\/jats:p>\n                  <jats:p>Contacts: andre.kahles@ratschlab.org or gunnar.ratsch@ratschlab.org<\/jats:p>\n                  <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btw076","type":"journal-article","created":{"date-parts":[[2016,2,14]],"date-time":"2016-02-14T20:09:07Z","timestamp":1455480547000},"page":"1840-1847","source":"Crossref","is-referenced-by-count":171,"title":["<i>SplAdder<\/i>\n                    : identification, quantification and testing of alternative splicing events from RNA-Seq data"],"prefix":"10.1093","volume":"32","author":[{"given":"Andr\u00e9","family":"Kahles","sequence":"first","affiliation":[{"name":"1 Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng Soon","family":"Ong","sequence":"additional","affiliation":[{"name":"2 Canberra Research Laboratory, NICTA, Canberra, ACT 2601, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhong","sequence":"additional","affiliation":[{"name":"1 Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnar","family":"R\u00e4tsch","sequence":"additional","affiliation":[{"name":"1 Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,2,11]]},"reference":[{"key":"2023020112293821000_btw076-B1","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1093\/bioinformatics\/btt442","article-title":"MITIE: Simultaneous RNA-Seq-based transcript identification and quantification in multiple samples","volume":"29","author":"Behr","year":"2013","journal-title":"Bioinformatics"},{"key":"2023020112293821000_btw076-B2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1101\/gr.108662.110","article-title":"Conservation of an RNA regulatory map between Drosophila and mammals","volume":"21","author":"Brooks","year":"2011","journal-title":"Genome Res"},{"key":"2023020112293821000_btw076-B3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"2023020112293821000_btw076-B4","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1105\/tpc.113.115485","article-title":"Nonsense-mediated decay of alternative precursor mRNA splicing variants is a major determinant of the arabidopsis steady state transcriptome","volume":"25","author":"Drechsel","year":"2013","journal-title":"Plant Cell"},{"key":"2023020112293821000_btw076-B5","doi-asserted-by":"crossref","first-page":"5189","DOI":"10.1093\/nar\/gkt211","article-title":"Accurate detection of differential RNA processing","volume":"41","author":"Drewe","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2023020112293821000_btw076-B6","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1471-2105-12-55","article-title":"Support vector machines-based identification of alternative splicing in Arabidopsis thaliana from whole-genome tiling arrays","volume":"12","author":"Eichner","year":"2011","journal-title":"BMC Bioinf"},{"key":"2023020112293821000_btw076-B7","doi-asserted-by":"crossref","first-page":"W297","DOI":"10.1093\/nar\/gkm311","article-title":"Astalavista: dynamic and flexible analysis of alternative splicing events in custom gene datasets","volume":"35","author":"Foissac","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"2023020112293821000_btw076-B8","first-page":"10249","article-title":"Multiple reference genomes and transcriptomes for Arabidopsis thaliana","volume":"108","author":"Gan","year":"2011","journal-title":"Nature"},{"key":"2023020112293821000_btw076-B9","doi-asserted-by":"crossref","first-page":"10073","DOI":"10.1093\/nar\/gks666","article-title":"Modelling and simulating generic RNA-Seq experiments with the flux simulator","volume":"40","author":"Griebel","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"2023020112293821000_btw076-B10","doi-asserted-by":"crossref","first-page":"e1000147","DOI":"10.1371\/journal.pcbi.1000147","article-title":"A general definition and nomenclature for alternative splicing events","volume":"4","author":"Guig\u00f3 Serra","year":"2008","journal-title":"PLoS Comput. 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