{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:14:25Z","timestamp":1775196865923,"version":"3.50.1"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Protein function depends on its structural stability. The effects of single point variations on protein stability can elucidate the molecular mechanisms of human diseases and help in developing new drugs. Recently, we introduced INPS, a method suited to predict the effect of variations on protein stability from protein sequence and whose performance is competitive with the available state-of-the-art tools.<\/jats:p>\n               <jats:p>Results: In this article, we describe INPS-MD (Impact of Non synonymous variations on Protein Stability-Multi-Dimension), a web server for the prediction of protein stability changes upon single point variation from protein sequence and\/or structure. Here, we complement INPS with a new predictor (INPS3D) that exploits features derived from protein 3D structure. INPS3D scores with Pearson\u2019s correlation to experimental \u0394\u0394G values of 0.58 in cross validation and of 0.72 on a blind test set. The sequence-based INPS scores slightly lower than the structure-based INPS3D and both on the same blind test sets well compare with the state-of-the-art methods.<\/jats:p>\n               <jats:p>Availability and Implementation: INPS and INPS3D are available at the same web server: http:\/\/inpsmd.biocomp.unibo.it.<\/jats:p>\n               <jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <jats:p>Contact: \u00a0gigi@biocomp.unibo.it<\/jats:p>","DOI":"10.1093\/bioinformatics\/btw192","type":"journal-article","created":{"date-parts":[[2016,4,12]],"date-time":"2016-04-12T01:13:10Z","timestamp":1460423590000},"page":"2542-2544","source":"Crossref","is-referenced-by-count":254,"title":["INPS-MD: a web server to predict stability of protein variants from sequence and structure"],"prefix":"10.1093","volume":"32","author":[{"given":"Castrense","family":"Savojardo","sequence":"first","affiliation":[{"name":"1 Biocomputing Group, CIG, Interdepartmental Center \u00abLuigi Galvani\u00bb for Integrated Studies of Bioinformatics, Biophysics and Biocomplexity, University of Bologna, Italy"}]},{"given":"Piero","family":"Fariselli","sequence":"additional","affiliation":[{"name":"2 BCA, University of Padova, Italy"}]},{"given":"Pier Luigi","family":"Martelli","sequence":"additional","affiliation":[{"name":"1 Biocomputing Group, CIG, Interdepartmental Center \u00abLuigi Galvani\u00bb for Integrated Studies of Bioinformatics, Biophysics and Biocomplexity, University of Bologna, Italy"}]},{"given":"Rita","family":"Casadio","sequence":"additional","affiliation":[{"name":"1 Biocomputing Group, CIG, Interdepartmental Center \u00abLuigi Galvani\u00bb for Integrated Studies of Bioinformatics, Biophysics and Biocomplexity, University of Bologna, Italy"},{"name":"3 Interdepartmental Center \u00abGiorgio Prodi\u00bb for Cancer Research, University of Bologna, Italy"}]}],"member":"286","published-online":{"date-parts":[[2016,4,10]]},"reference":[{"key":"2023020112524802500_btw192-B1","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1001\/jama.2015.3595","article-title":"The precision medicine initiative: a new national effort","volume":"313","author":"Ashley","year":"2015","journal-title":"JAMA"},{"key":"2023020112524802500_btw192-B2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/prot.1075","article-title":"How to guarantee optimal stability for most representative structures in the protein data bank","volume":"44","author":"Bastolla","year":"2001","journal-title":"Proteins"},{"key":"2023020112524802500_btw192-B3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1145\/1961189.1961199","article-title":"LIBSVM: a library for support vector machine","volume":"2","author":"Chang","year":"2011","journal-title":"ACM Transactions on Intel. 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