{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,6]],"date-time":"2025-04-06T21:43:01Z","timestamp":1743975781537},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"16","license":[{"start":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T00:00:00Z","timestamp":1477612800000},"content-version":"vor","delay-in-days":198,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: In many organisms, including humans, recombination clusters within recombination hotspots. The standard method for de novo detection of recombinants at hotspots is sperm typing. This relies on allele-specific PCR at single nucleotide polymorphisms. Designing allele-specific primers by hand is time-consuming. We have therefore written a package to support hotspot detection and analysis.<\/jats:p>\n               <jats:p>Results: hotspot consists of four programs: asp looks up SNPs and designs allele-specific primers; aso constructs allele-specific oligos for mapping recombinants; xov implements a maximum-likelihood method for estimating the crossover rate; six, finally, simulates typing data.<\/jats:p>\n               <jats:p>Availability and Implementation: hotspot is written in C. Sources are freely available under the GNU General Public License from http:\/\/github.com\/evolbioinf\/hotspot\/<\/jats:p>\n               <jats:p>Contact: \u00a0haubold@evolbio.mpg.de<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btw195","type":"journal-article","created":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T01:25:59Z","timestamp":1460597159000},"page":"2554-2555","source":"Crossref","is-referenced-by-count":2,"title":["hotspot: software to support sperm-typing for investigating recombination hotspots"],"prefix":"10.1093","volume":"32","author":[{"given":"Linda","family":"Odenthal-Hesse","sequence":"first","affiliation":[{"name":"Department of Evolutionary Genetics, Max-Planck-Institute for Evolutionary Biology, Pl\u00f6n, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien Y.","family":"Dutheil","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Genetics, Max-Planck-Institute for Evolutionary Biology, Pl\u00f6n, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabian","family":"Kl\u00f6tzl","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Genetics, Max-Planck-Institute for Evolutionary Biology, Pl\u00f6n, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"Haubold","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Genetics, Max-Planck-Institute for Evolutionary Biology, Pl\u00f6n, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,4,13]]},"reference":[{"key":"2023020112533080100_btw195-B1","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nature10336","article-title":"The landscape of recombination in African Americans","volume":"476","author":"Hinch","year":"2011","journal-title":"Nature"},{"key":"2023020112533080100_btw195-B2","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/ng1565","article-title":"Human recombination hot spots hidden in regions of strong marker association","volume":"37","author":"Jeffreys","year":"2005","journal-title":"Nat. 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