{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T01:35:59Z","timestamp":1769304959851,"version":"3.49.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T00:00:00Z","timestamp":1471305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/about_us\/legal\/notices"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>Successful development and application of precision oncology approaches require robust elucidation of the genomic landscape of a patient\u2019s cancer and, ideally, the ability to monitor therapy-induced genomic changes in the tumour in an inexpensive and minimally invasive manner. Thanks to recent advances in sequencing technologies, \u2018liquid biopsy\u2019, the sampling of patient\u2019s bodily fluids such as blood and urine, is considered as one of the most promising approaches to achieve this goal. In many cancer patients, and especially those with advanced metastatic disease, deep sequencing of circulating cell free DNA (cfDNA) obtained from patient\u2019s blood yields a mixture of reads originating from the normal DNA and from multiple tumour subclones\u2014called circulating tumour DNA or ctDNA. The ctDNA\/cfDNA ratio as well as the proportion of ctDNA originating from specific tumour subclones depend on multiple factors, making comprehensive detection of mutations difficult, especially at early stages of cancer. Furthermore, sensitive and accurate detection of single nucleotide variants (SNVs) and indels from cfDNA is constrained by several factors such as the sequencing errors and PCR artifacts, and mapping errors related to repeat regions within the genome. In this article, we introduce SiNVICT, a computational method that increases the sensitivity and specificity of SNV and indel detection at very low variant allele frequencies. SiNVICT has the capability to handle multiple sequencing platforms with different error properties; it minimizes false positives resulting from mapping errors and other technology specific artifacts including strand bias and low base quality at read ends. SiNVICT also has the capability to perform time-series analysis, where samples from a patient sequenced at multiple time points are jointly examined to report locations of interest where there is a possibility that certain clones were wiped out by some treatment while some subclones gained selective advantage.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We tested SiNVICT on simulated data as well as prostate cancer cell lines and cfDNA obtained from castration-resistant prostate cancer patients. On both simulated and biological data, SiNVICT was able to detect SNVs and indels with variant allele percentages as low as 0.5%. The lowest amounts of total DNA used for the biological data where SNVs and indels could be detected with very high sensitivity were 2.5\u2009ng on the Ion Torrent platform and 10\u2009ng on Illumina. With increased sequencing and mapping accuracy, SiNVICT might be utilized in clinical settings, making it possible to track the progress of point mutations and indels that are associated with resistance to cancer therapies and provide patients personalized treatment. We also compared SiNVICT with other popular SNV callers such as MuTect, VarScan2 and Freebayes. Our results show that SiNVICT performs better than these tools in most cases and allows further data exploration such as time-series analysis on cfDNA sequencing data.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and Implementation<\/jats:title>\n                  <jats:p>SiNVICT is available at: https:\/\/sfu-compbio.github.io\/sinvict<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btw536","type":"journal-article","created":{"date-parts":[[2016,8,17]],"date-time":"2016-08-17T02:39:40Z","timestamp":1471401580000},"page":"26-34","source":"Crossref","is-referenced-by-count":57,"title":["SiNVICT: ultra-sensitive detection of single nucleotide variants and indels in circulating tumour DNA"],"prefix":"10.1093","volume":"33","author":[{"given":"Can","family":"Kockan","sequence":"first","affiliation":[{"name":"School of Computing Science, Simon Fraser University, Burnaby, (BC), Canada"},{"name":"MADD-Gen Graduate Program, Simon Fraser University, Burnaby, (BC), Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faraz","family":"Hach","sequence":"additional","affiliation":[{"name":"School of Computing Science, Simon Fraser University, Burnaby, (BC), Canada"},{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iman","family":"Sarrafi","sequence":"additional","affiliation":[{"name":"School of Computing Science, Simon Fraser University, Burnaby, (BC), Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H","family":"Bell","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"McConeghy","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Beja","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Haegert","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander W","family":"Wyatt","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"},{"name":"Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanislav V","family":"Volik","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim N","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin C","family":"Collins","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre, Vancouver, BC, Canada"},{"name":"Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S Cenk","family":"Sahinalp","sequence":"additional","affiliation":[{"name":"School of Computing Science, Simon Fraser University, Burnaby, (BC), Canada"},{"name":"Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada"},{"name":"School of Informatics and Computing, Indiana University, Bloomington, IN, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,8,16]]},"reference":[{"key":"2023020204301821400_btw536-B1","doi-asserted-by":"crossref","first-page":"C89","DOI":"10.1158\/1535-7163.TARG-13-C89","article-title":"Abstract c89: Galeterone suppresses castration-resistant and enzalutamide-resistant prostate cancer growth in vitro","volume":"12","author":"Al Nakouzi","year":"2013","journal-title":"Mol. 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