{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:04:32Z","timestamp":1773270272452,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"24","funder":[{"DOI":"10.13039\/501100013286","name":"Specialized Research Fund for the Doctoral Program of Higher Education","doi-asserted-by":"crossref","award":["20130031120001"],"award-info":[{"award-number":["20130031120001"]}],"id":[{"id":"10.13039\/501100013286","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Health and Medical Research Council of Australia","award":["1059775 and 1083450"],"award-info":[{"award-number":["1059775 and 1083450"]}]},{"name":"Australian Research Council\u2019s Linkage Infrastructure, Equipment and Facilities","award":["LE150100161"],"award-info":[{"award-number":["LE150100161"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,12,15]]},"abstract":"<jats:p>Motivation: Backbone structures and solvent accessible surface area of proteins are benefited from continuous real value prediction because it removes the arbitrariness of defining boundary between different secondary-structure and solvent-accessibility states. However, lacking the confidence score for predicted values has limited their applications. Here we investigated whether or not we can make a reasonable prediction of absolute errors for predicted backbone torsion angles, C\u03b1-atom-based angles and torsion angles, solvent accessibility, contact numbers and half-sphere exposures by employing deep neural networks.<\/jats:p><jats:p>Results: We found that angle-based errors can be predicted most accurately with Spearman correlation coefficient (SPC) between predicted and actual errors at about 0.6. This is followed by solvent accessibility (SPC\u223c0.5). The errors on contact-based structural properties are most difficult to predict (SPC between 0.2 and 0.3). We showed that predicted errors are significantly better error indicators than the average errors based on secondary-structure and amino-acid residue types. We further demonstrated the usefulness of predicted errors in model quality assessment. These error or confidence indictors are expected to be useful for prediction, assessment, and refinement of protein structures.<\/jats:p><jats:p>Availability and Implementation: The method is available at http:\/\/sparks-lab.org as a part of SPIDER2 package.<\/jats:p><jats:p>Contact: \u00a0yuedong.yang@griffith.edu.au or yaoqi.zhou@griffith.edu.au<\/jats:p><jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btw549","type":"journal-article","created":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T00:28:57Z","timestamp":1471912137000},"page":"3768-3773","source":"Crossref","is-referenced-by-count":24,"title":["Predicting the errors of predicted local backbone angles and non-local solvent- accessibilities of proteins by deep neural networks"],"prefix":"10.1093","volume":"32","author":[{"given":"Jianzhao","family":"Gao","sequence":"first","affiliation":[{"name":"1School of Mathematical Sciences and LPMC, Nankai University, Tianjin, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuedong","family":"Yang","sequence":"additional","affiliation":[{"name":"2Institute for Glycomics and School of Information and Communication Technology, Griffith University, Parklands Dr, Southport, QLD 4222, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"2Institute for Glycomics and School of Information and Communication Technology, Griffith University, Parklands Dr, Southport, QLD 4222, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,8,22]]},"reference":[{"key":"2023020114075957900_btw549-B1","doi-asserted-by":"crossref","first-page":"456","DOI":"10.2174\/1389203715666140327114232","article-title":"A review of methods available to estimate solvent-accessible surface areas of soluble proteins in the folded and unfolded states","volume":"15","author":"Ali","year":"2014","journal-title":"Curr. 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