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We have applied it to mapping DNA reads from IonTorrent and PacBio RS, and we show that it reduces reference bias for Oxford Nanopore reads.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and Implementation<\/jats:title>\n                  <jats:p>the source code is freely available at http:\/\/last.cbrc.jp\/<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btw742","type":"journal-article","created":{"date-parts":[[2016,11,19]],"date-time":"2016-11-19T04:05:52Z","timestamp":1479528352000},"page":"926-928","source":"Crossref","is-referenced-by-count":89,"title":["Training alignment parameters for arbitrary sequencers with LAST-TRAIN"],"prefix":"10.1093","volume":"33","author":[{"given":"Michiaki","family":"Hamada","sequence":"first","affiliation":[{"name":"Department of Electrical Engineering and Bioscience, Faculty of Science and Engineering, Waseda University, 55N\u201306\u201310, 3\u20134\u20131, Okubo Shinjuku-ku, Tokyo, Japan"},{"name":"Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan"},{"name":"Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiteru","family":"Ono","sequence":"additional","affiliation":[{"name":"IMSBIO Co, Ltd, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Asai","sequence":"additional","affiliation":[{"name":"Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan"},{"name":"Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin C","family":"Frith","sequence":"additional","affiliation":[{"name":"Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan"},{"name":"Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan"},{"name":"Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,11,23]]},"reference":[{"key":"2023020204521268700_btw742-B1","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"2023020204521268700_btw742-B2","doi-asserted-by":"crossref","first-page":"17.","DOI":"10.12688\/f1000research.6037.2","article-title":"Long read nanopore sequencing for detection of HLA and CYP2D6 variants and haplotypes","volume":"4","author":"Ammar","year":"2015","journal-title":"F1000Res"},{"key":"2023020204521268700_btw742-B3","doi-asserted-by":"crossref","first-page":"238.","DOI":"10.1186\/1471-2105-13-238","article-title":"Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory","volume":"13","author":"Chaisson","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"2023020204521268700_btw742-B4","first-page":"115","article-title":"Scoring pairwise genomic sequence alignments","author":"Chiaromonte","year":"2002","journal-title":"Pac. 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