{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T07:52:33Z","timestamp":1781250753589,"version":"3.54.1"},"reference-count":5,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T00:00:00Z","timestamp":1485475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/about_us\/legal\/notices"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1062470"],"award-info":[{"award-number":["APP1062470"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>Spike-in controls are synthetic nucleic-acid sequences that are added to a user\u2019s sample and constitute internal standards for subsequent steps in the next generation sequencing workflow.<\/jats:p>\n                  <jats:p>The Anaquin software toolkit can be used to analyze the performance of spike-in controls at multiple steps during RNA sequencing or genome sequencing analysis, providing useful diagnostic statistics, data visualization and sample normalization.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and Implementation<\/jats:title>\n                  <jats:p>The software is implemented in C\u2009++\/R and is freely available under BSD license. The source code is available from github.com\/student-t\/Anaquin, binaries and user manual from www.sequin.xyz\/software and R package from bioconductor.org\/packages\/Anaquin<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btx038","type":"journal-article","created":{"date-parts":[[2017,1,28]],"date-time":"2017-01-28T01:25:31Z","timestamp":1485566731000},"page":"1723-1724","source":"Crossref","is-referenced-by-count":17,"title":["ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing"],"prefix":"10.1093","volume":"33","author":[{"given":"Ted","family":"Wong","sequence":"first","affiliation":[{"name":"Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira W","family":"Deveson","sequence":"additional","affiliation":[{"name":"Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia"},{"name":"Faculty of Science, School of Biotechnology and Biomolecular Sciences, UNSW, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon A","family":"Hardwick","sequence":"additional","affiliation":[{"name":"Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia"},{"name":"Faculty of Medicine, St Vincents Clinical School, UNSW, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim R","family":"Mercer","sequence":"additional","affiliation":[{"name":"Genomics and Epigenetics Division, Garvan Institute of Medical Research, Sydney, NSW, Australia"},{"name":"Faculty of Medicine, St Vincents Clinical School, UNSW, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,1,27]]},"reference":[{"key":"2023020205304232300_btx038-B1","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1038\/nmeth.3957","article-title":"Representing genetic variation with synthetic DNA standards","volume":"13","author":"Deveson","year":"2016","journal-title":"Nat. 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