{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:23:03Z","timestamp":1762917783851,"version":"3.37.3"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"15","license":[{"start":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T00:00:00Z","timestamp":1490745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB-1330800","DUE-132809"],"award-info":[{"award-number":["MCB-1330800","DUE-132809"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>The Sequence Read Archive (SRA) contains raw data from many different types of sequence projects. As of 2017, the SRA contained approximately ten petabases of DNA sequence (1016 bp). Annotations of the data are provided by the submitter, and mining the data in the SRA is complicated by both the amount of data and the detail within those annotations. Here, we introduce PARTIE, a partition engine optimized to differentiate sequence read data into metagenomic (random) and amplicon (targeted) sequence data sets.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>PARTIE subsamples reads from the sequencing file and calculates four different statistics: k-mer frequency, 16S abundance, prokaryotic- and viral-read abundance. These metrics are used to create a RandomForest decision tree to classify the sequencing data, and PARTIE provides mechanisms for both supervised and unsupervised classification. We demonstrate the accuracy of PARTIE for classifying SRA data, discuss the probable error rates in the SRA annotations and introduce a resource assessing SRA data.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and Implementation<\/jats:title>\n                  <jats:p>PARTIE and reclassified metagenome SRA entries are available from https:\/\/github.com\/linsalrob\/partie<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btx184","type":"journal-article","created":{"date-parts":[[2017,3,28]],"date-time":"2017-03-28T20:07:11Z","timestamp":1490731631000},"page":"2389-2391","source":"Crossref","is-referenced-by-count":26,"title":["PARTIE: a partition engine to separate metagenomic and amplicon projects in the Sequence Read Archive"],"prefix":"10.1093","volume":"33","author":[{"given":"Pedro J","family":"Torres","sequence":"first","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA, USA"}]},{"given":"Robert A","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA, USA"},{"name":"Computational Science Research Center, San Diego State University, San Diego, CA, USA"},{"name":"Department of Computer Science, San Diego State University, San Diego, CA, USA"}]},{"given":"Katelyn A","family":"McNair","sequence":"additional","affiliation":[{"name":"Computational Science Research Center, San Diego State University, San Diego, CA, USA"}]}],"member":"286","published-online":{"date-parts":[[2017,3,30]]},"reference":[{"key":"2023063012503686900_btx184-B1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST Server: rapid annotations using subsystems technology","volume":"9","author":"Aziz","year":"2008","journal-title":"BMC Genomics"},{"key":"2023063012503686900_btx184-B2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1023\/A:1010933404324","article-title":"Random forests","volume":"45","author":"Breiman","year":"2001","journal-title":"Mach. 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