{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T07:49:42Z","timestamp":1773128982642,"version":"3.50.1"},"reference-count":3,"publisher":"Oxford University Press (OUP)","issue":"23","license":[{"start":{"date-parts":[[2017,7,13]],"date-time":"2017-07-13T00:00:00Z","timestamp":1499904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/about_us\/legal\/notices"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["16-34272A"],"award-info":[{"award-number":["16-34272A"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>Sanger sequencing is still being employed for sequence variant detection by many laboratories, especially in a clinical setting. However, chromatogram interpretation often requires manual inspection and in some cases, considerable expertise.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We present GLASS, a web-based Sanger sequence trace viewer, editor, aligner and variant caller, built to assist with the assessment of variations in \u2018curated\u2019 or user-provided genes. Critically, it produces a standardized variant output as recommended by the Human Genome Variation Society.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>GLASS is freely available at http:\/\/bat.infspire.org\/genomepd\/glass\/ with source code at https:\/\/github.com\/infspiredBAT\/GLASS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Supplementary information<\/jats:title>\n                    <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btx423","type":"journal-article","created":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T07:20:01Z","timestamp":1499844001000},"page":"3802-3804","source":"Crossref","is-referenced-by-count":11,"title":["GLASS: assisted and standardized assessment of gene variations from Sanger sequence trace data"],"prefix":"10.1093","volume":"33","author":[{"given":"Karol","family":"Pal","sequence":"first","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vojtech","family":"Bystry","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Reigl","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Demko","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Krejci","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tasoula","family":"Touloumenidou","sequence":"additional","affiliation":[{"name":"Hematology Department and HCT Unit, G. Papanicolaou Hospital, Center for Research and Technology Hellas, Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelia","family":"Stalika","sequence":"additional","affiliation":[{"name":"Institute of Applied Biosciences, Center for Research and Technology Hellas, Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Tichy","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Ghia","sequence":"additional","affiliation":[{"name":"Division of Molecular Oncology, Department of Onco-Hematology, IRCCS San Raffaele Scientific Institute, Universit\u00e0 Vita-Salute San Raffaele, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kostas","family":"Stamatopoulos","sequence":"additional","affiliation":[{"name":"Institute of Applied Biosciences, Center for Research and Technology Hellas, Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarka","family":"Pospisilova","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"},{"name":"Department of Internal Medicine--Hematology and Oncology, University Hospital Brno, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitka","family":"Malcikova","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"},{"name":"Department of Internal Medicine--Hematology and Oncology, University Hospital Brno, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikos","family":"Darzentas","sequence":"additional","affiliation":[{"name":"CEITEC\u2014Central European Institute of Technology, Masaryk University, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"European Research Initiative on CLL (ERIC)\u2014TP53 Network","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,7,13]]},"reference":[{"key":"2023020207004066000_btx423-B1","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1109\/TVCG.2011.185","article-title":"D3: Data-Driven Documents","volume":"17","author":"Bostock","year":"2011","journal-title":"IEEE Trans Vis. 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