{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:26:48Z","timestamp":1780709208362,"version":"3.54.1"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"16","license":[{"start":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T00:00:00Z","timestamp":1546819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Office for the Advancement of Research and Scholarship"},{"name":"OARS"},{"name":"Biology Department, Miami University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cpf1 system has been successfully applied in genome editing. However, target efficiency of the CRISPR-Cpf1 system varies among different guide RNA (gRNA) sequences.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In this study, we reanalyzed the published CRISPR-Cpf1 gRNAs data and found many sequence and structural features related to their target efficiency. With the aid of Random Forest in feature selection, a support vector machine model was created to predict target efficiency for any given gRNAs. We have developed the first CRISPR-Cpf1 web service application, CRISPR-DT (CRISPR DNA Targeting), to help users design optimal gRNAs for the CRISPR-Cpf1 system by considering both target efficiency and specificity. CRISPR-DT will empower researchers in genome editing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>CRISPR-DT, mainly implemented in Perl, PHP and JavaScript, is freely available at http:\/\/bioinfolab.miamioh.edu\/CRISPR-DT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Supplementary information<\/jats:title>\n                    <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/bty1061","type":"journal-article","created":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T15:09:26Z","timestamp":1546441766000},"page":"2783-2789","source":"Crossref","is-referenced-by-count":72,"title":["CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity"],"prefix":"10.1093","volume":"35","author":[{"given":"Houxiang","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Biology, Miami University, Oxford, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Biology, Miami University, Oxford, OH, USA"},{"name":"Department of Computer Science and Software Engineering, Miami University, Oxford, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,1,7]]},"reference":[{"key":"2023062708582480300_bty1061-B1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1101\/gr.161638.113","article-title":"Highly efficient CRISPR\/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair","volume":"24","author":"Auer","year":"2014","journal-title":"Genome Res"},{"key":"2023062708582480300_bty1061-B2","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.celrep.2013.06.020","article-title":"Highly efficient targeted mutagenesis of drosophila with the CRISPR\/Cas9 System","volume":"4","author":"Bassett","year":"2013","journal-title":"Cell Rep"},{"key":"2023062708582480300_bty1061-B3","doi-asserted-by":"crossref","first-page":"27.","DOI":"10.1145\/1961189.1961199","article-title":"LIBSVM: a library for support vector machines","volume":"2","author":"Chang","year":"2011","journal-title":"ACM Trans. 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