{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T12:29:09Z","timestamp":1779107349997,"version":"3.51.4"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"19","license":[{"start":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T00:00:00Z","timestamp":1525219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council","doi-asserted-by":"crossref","award":["RGPIN-2014-06664"],"award-info":[{"award-number":["RGPIN-2014-06664"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI126466"],"award-info":[{"award-number":["R21AI126466"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"SickKids RestraComp scholarship"},{"name":"SciNet HPC Consortium"},{"name":"SciNet"},{"name":"Canada Foundation"},{"DOI":"10.13039\/100013873","name":"Government of Ontario","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100013873","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ontario Research Fund\u2013Research Excellence"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>We present DETECT v2\u2014an enzyme annotation tool which considers the effect of sequence diversity when assigning enzymatic function [as an Enzyme Commission (EC) number] to a protein sequence. In addition to capturing more enzyme classes than the previous version, we now provide EC-specific cutoffs that greatly increase precision and recall of assignments and show its performance in the context of pathways.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>https:\/\/github.com\/ParkinsonLab\/DETECT-v2<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/bty368","type":"journal-article","created":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T19:09:42Z","timestamp":1525201782000},"page":"3393-3395","source":"Crossref","is-referenced-by-count":22,"title":["Improved enzyme annotation with EC-specific cutoffs using DETECT v2"],"prefix":"10.1093","volume":"34","author":[{"given":"Nirvana","family":"Nursimulu","sequence":"first","affiliation":[{"name":"Program in Molecular Medicine, The Hospital for Sick Children, 21\u20139709 PGCRL, 686 Bay Street, Toronto, ON, Canada"},{"name":"Department of Computer Science, University of Toronto, Toronto, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leon L","family":"Xu","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, The Hospital for Sick Children, 21\u20139709 PGCRL, 686 Bay Street, Toronto, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James D","family":"Wasmuth","sequence":"additional","affiliation":[{"name":"Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Krukov","sequence":"additional","affiliation":[{"name":"Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Parkinson","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, The Hospital for Sick Children, 21\u20139709 PGCRL, 686 Bay Street, Toronto, ON, Canada"},{"name":"Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada"},{"name":"Department of Biochemistry, University of Toronto, Toronto, ON, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,5,2]]},"reference":[{"key":"2023012712485253100_bty368-B1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. 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