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To build up accurate information about biological structures, it is critical that the quantification is both accurate (close to the ground truth) and precise (has small scatter and is reproducible).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Here, we describe how the R\u00e9nyi divergence can be used for cluster radius measurements in localization microscopy data. We demonstrate that the R\u00e9nyi divergence can operate with high levels of background and provides results which are more accurate than Ripley\u2019s functions, Voronoi tesselation or DBSCAN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Availability and implementation<\/jats:title><jats:p>The data supporting this research and the software described are accessible at the following site: https:\/\/dx.doi.org\/10.18742\/RDM01-316. Correspondence and requests for materials should be addressed to the corresponding author.<\/jats:p><\/jats:sec><jats:sec><jats:title>Supplementary information<\/jats:title><jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p><\/jats:sec>","DOI":"10.1093\/bioinformatics\/bty403","type":"journal-article","created":{"date-parts":[[2018,5,31]],"date-time":"2018-05-31T03:42:39Z","timestamp":1527738159000},"page":"4102-4111","source":"Crossref","is-referenced-by-count":6,"title":["The R\u00e9nyi divergence enables accurate and precise cluster analysis for localization microscopy"],"prefix":"10.1093","volume":"34","author":[{"given":"Adela D","family":"Staszowska","sequence":"first","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, UK"}]},{"given":"Patrick","family":"Fox-Roberts","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, UK"}]},{"given":"Liisa M","family":"Hirvonen","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, UK"}]},{"given":"Christopher J","family":"Peddie","sequence":"additional","affiliation":[{"name":"Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, UK"}]},{"given":"Lucy M","family":"Collinson","sequence":"additional","affiliation":[{"name":"Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, UK"}]},{"given":"Gareth E","family":"Jones","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, UK"}]},{"given":"Susan","family":"Cox","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, UK"}]}],"member":"286","published-online":{"date-parts":[[2018,6,1]]},"reference":[{"key":"2023012712285576200_bty403-B1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1145\/304181.304187","article-title":"OPTICS: ordering points to identify the clustering structure","volume":"28","author":"Ankers","year":"1999","journal-title":"Proc. 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