{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:36:06Z","timestamp":1788381366160,"version":"build-2803163510"},"reference-count":78,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013060","name":"European Molecular Biology Laboratory","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Future Leader Fellowship","award":["BB\/N011600\/1"],"award-info":[{"award-number":["BB\/N011600\/1"]}]},{"name":"Spanish Ministry of Economy and Competitiveness"},{"DOI":"10.13039\/501100003329","name":"MINECO","doi-asserted-by":"publisher","award":["BIO2016-79930-R"],"award-info":[{"award-number":["BIO2016-79930-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Interreg POCTEFA","award":["EFA086\/15"],"award-info":[{"award-number":["EFA086\/15"]}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["676566"],"award-info":[{"award-number":["676566"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>Understanding the relationship between the sequence, structure, binding energy, binding kinetics and binding thermodynamics of protein\u2013protein interactions is crucial to understanding cellular signaling, the assembly and regulation of molecular complexes, the mechanisms through which mutations lead to disease, and protein engineering.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We present SKEMPI 2.0, a major update to our database of binding free energy changes upon mutation for structurally resolved protein\u2013protein interactions. This version now contains manually curated binding data for 7085 mutations, an increase of 133%, including changes in kinetics for 1844 mutations, enthalpy and entropy changes for 443 mutations, and 440 mutations, which abolish detectable binding.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>The database is available as supplementary data and at https:\/\/life.bsc.es\/pid\/skempi2\/.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Supplementary information<\/jats:title>\n                    <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/bty635","type":"journal-article","created":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T13:05:03Z","timestamp":1531919103000},"page":"462-469","source":"Crossref","is-referenced-by-count":358,"title":["SKEMPI 2.0: an updated benchmark of changes in protein\u2013protein binding energy, kinetics and thermodynamics upon mutation"],"prefix":"10.1093","volume":"35","author":[{"given":"Justina","family":"Jankauskait\u0117","sequence":"first","affiliation":[{"name":"Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Jim\u00e9nez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Barcelona Supercomputing Center (BSC), Barcelona, Spain"},{"name":"Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University, Utrecht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justas","family":"Dapk\u016bnas","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Fern\u00e1ndez-Recio","sequence":"additional","affiliation":[{"name":"Barcelona Supercomputing Center (BSC), Barcelona, Spain"},{"name":"Institut de Biologia Molecular de Barcelona (IBMB), CSIC, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4960-5487","authenticated-orcid":false,"given":"Iain H","family":"Moal","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,7,18]]},"reference":[{"key":"2023013107240909400_bty635-B1","doi-asserted-by":"crossref","first-page":"e1003216","DOI":"10.1371\/journal.pcbi.1003216","article-title":"Characterizing changes in the rate of protein-protein dissociation upon interface mutation using hotspot energy and organization","volume":"9","author":"Agius","year":"2013","journal-title":"PLoS Comput. 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