{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T02:04:19Z","timestamp":1762999459147,"version":"3.37.3"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"18","license":[{"start":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T00:00:00Z","timestamp":1549411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Grants-in-Aid for Scientific Research on Innovative Areas \u2018Platform of Advanced Animal Model Support\u2019","award":["16H06276A"],"award-info":[{"award-number":["16H06276A"]}]},{"name":"Project for Elucidating and Controlling Mechanisms of Aging and Longevity"},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["JP18gm501003s1102"],"award-info":[{"award-number":["JP18gm501003s1102"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>Although gene editing using the CRISPR\/Cas9 system enables the rapid generation of knockout mice, constructing an optimal gene disruption strategy is still labourious. Here, we propose KOnezumi, a simple and user-friendly web application, for use in automating the design of knockout strategies for multiple genes. Users only need to input gene symbols, and then KOnezumi returns target exons, gRNA candidates to delete the target exons, genotyping PCR primers, nucleotide sequences of the target exons and coding sequences of expected deletion products. KOnezumi enables users to easily and rapidly apply a rational strategy to accelerate the generation of KO mice using the CRISPR\/Cas9 system.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>This web application is freely available at http:\/\/www.md.tsukuba.ac.jp\/LabAnimalResCNT\/KOanimals\/konezumi.html.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btz090","type":"journal-article","created":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T12:33:02Z","timestamp":1549369982000},"page":"3479-3481","source":"Crossref","is-referenced-by-count":7,"title":["KOnezumi: a web application for automating gene disruption strategies to generate knockout mice"],"prefix":"10.1093","volume":"35","author":[{"given":"Akihiro","family":"Kuno","sequence":"first","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Medicine , Tsukuba, Ibaraki, Japan"}]},{"given":"Seiya","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center, University of Tsukuba , Tsukuba, Ibaraki, Japan"}]},{"given":"Satoru","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Medicine , Tsukuba, Ibaraki, Japan"},{"name":"Laboratory Animal Resource Center, University of Tsukuba , Tsukuba, Ibaraki, Japan"}]}],"member":"286","published-online":{"date-parts":[[2019,2,6]]},"reference":[{"key":"2023013108051817100_btz090-B1","doi-asserted-by":"crossref","first-page":"W242","DOI":"10.1093\/nar\/gky354","article-title":"CRISPOR: intuitive guide selection for CRISPR\/Cas9 genome editing experiments and screens","volume":"46","author":"Concordet","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"2023013108051817100_btz090-B2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1038\/nbt.3437","article-title":"Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9","volume":"34","author":"Doench","year":"2016","journal-title":"Nat. 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