{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T07:57:42Z","timestamp":1769155062821,"version":"3.49.0"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"18","license":[{"start":{"date-parts":[[2019,2,19]],"date-time":"2019-02-19T00:00:00Z","timestamp":1550534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100019018","name":"CAMS Initiative for Innovative Medicine","doi-asserted-by":"crossref","award":["2017-12M-1-015"],"award-info":[{"award-number":["2017-12M-1-015"]}],"id":[{"id":"10.13039\/501100019018","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100019018","name":"CAMS Initiative for Innovative Medicine","doi-asserted-by":"crossref","award":["2017-I2M-3-009"],"award-info":[{"award-number":["2017-I2M-3-009"]}],"id":[{"id":"10.13039\/501100019018","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds for the Central Research Institutes","award":["2017PT31034"],"award-info":[{"award-number":["2017PT31034"]}]},{"name":"the National Key Research and Development Program of China","award":["2018YFA0107804"],"award-info":[{"award-number":["2018YFA0107804"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81890993"],"award-info":[{"award-number":["81890993"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>Single-cell bisulfite sequencing (BS-seq) techniques have been developed for DNA methylation heterogeneity detection and studies with limited materials. However, the data deficiency such as low read mapping ratio is still a critical issue.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We comprehensively characterize single-cell BS-seq data and reveal chimerical molecules to be the major source of alignment failures. These chimerical molecules are produced by recombination of genomic proximal sequences with microhomology regions (MR) after bisulfite conversion. In addition, we find DNA methylation within MR is highly variable, suggesting the necessity of removing these regions to accurately estimate DNA methylation levels. We further develop scBS-map to perform quality control and local alignment of bisulfite sequencing data, chimerical molecule determination and MR removal. Using scBS-map, we show remarkable increases in uniquely mapped reads, genomic coverage and number of CpG sites, and recover more functional elements with precise DNA methylation estimation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>The scBS-map software is freely available at https:\/\/github.com\/wupengomics\/scBS-map.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btz125","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T04:25:53Z","timestamp":1550463953000},"page":"3273-3278","source":"Crossref","is-referenced-by-count":25,"title":["Using local alignment to enhance single-cell bisulfite sequencing data efficiency"],"prefix":"10.1093","volume":"35","author":[{"given":"Peng","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , Tianjin, China"},{"name":"Center for Stem Cell Medicine, Chinese Academy of Medical Sciences , Tianjin, China"},{"name":"Department of Stem Cell & Regenerative Medicine, Peking Union Medical College , Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Center for Breast Cancer Prevention and TherapyTianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer , Tianjin, China"},{"name":"Key Laboratory of Cancer Prevention and Therapy , Tianjin, China"},{"name":"Tianjin\u2019s Clinical Research Center for Cancer , Tianjin, 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