{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T08:09:23Z","timestamp":1778227763280,"version":"3.51.4"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T00:00:00Z","timestamp":1560902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100008333","name":"University of Alabama at Birmingham","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008333","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006545","name":"National Institute of Minority Health and Health Disparities","doi-asserted-by":"crossref","award":["U54MD000502"],"award-info":[{"award-number":["U54MD000502"]}],"id":[{"id":"10.13039\/100006545","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"publisher","award":["3U01HG007301-06S1"],"award-info":[{"award-number":["3U01HG007301-06S1"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institute for Precision Cardiovascular Medicine"},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"AHA","doi-asserted-by":"publisher","award":["17IF33890015"],"award-info":[{"award-number":["17IF33890015"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The UAB Center for Clinical and Translational Science"},{"DOI":"10.13039\/100010573","name":"CCTS","doi-asserted-by":"publisher","award":["UL1 TR001417"],"award-info":[{"award-number":["UL1 TR001417"]}],"id":[{"id":"10.13039\/100010573","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>Meiotic recombination facilitates the transmission of exchanged genetic material between homologous chromosomes and plays a crucial role in increasing the genetic variations in eukaryotic organisms. In humans, thousands of crossover events have been identified by genotyping related family members. However, most of these crossover regions span tens to hundreds of kb, which is not sufficient resolution to accurately identify the crossover breakpoints in a typical trio family.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We have developed MRLR, a software using 10X linked reads to identify crossover events at a high resolution. By reconstructing the gamete genome, MRLR only requires a trio family dataset and can efficiently discover the crossover events. Using MRLR, we revealed a fine-scale pattern of crossover regions in six human families. From the two closest heterozygous alleles around the crossovers, we determined that MRLR achieved a median resolution 4.5\u00a0kb. This method can delineate a genome-wide landscape of crossover events at a precise scale, which is important for both functional and genomic features analysis of meiotic recombination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>MRLR is freely available at https:\/\/github.com\/ChongLab\/MRLR, implemented in Perl.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Supplementary information<\/jats:title>\n                    <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btz503","type":"journal-article","created":{"date-parts":[[2019,6,12]],"date-time":"2019-06-12T15:12:56Z","timestamp":1560352376000},"page":"10-16","source":"Crossref","is-referenced-by-count":9,"title":["MRLR: unraveling high-resolution meiotic recombination by linked reads"],"prefix":"10.1093","volume":"36","author":[{"given":"Peng","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Genetics, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294,","place":["USA"]},{"name":"Informatics Institute, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Timothy","family":"Kennell","sequence":"additional","affiliation":[{"name":"Informatics Institute, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Gao","sequence":"additional","affiliation":[{"name":"Informatics Institute, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Human Genome Structural Variation Consortium","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert P","family":"Kimberly","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5750-1808","authenticated-orcid":false,"given":"Zechen","family":"Chong","sequence":"additional","affiliation":[{"name":"Department of Genetics, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294,","place":["USA"]},{"name":"Informatics Institute, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL 35294,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,6,19]]},"reference":[{"key":"2026041420021941700_btz503-B1","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1101\/gr.170167.113","article-title":"PRDM9 binding organizes hotspot nucleosomes and limits Holliday junction migration","volume":"24","author":"Baker","year":"2014","journal-title":"Genome Res"},{"key":"2026041420021941700_btz503-B2","doi-asserted-by":"crossref","first-page":"e162","DOI":"10.1093\/nar\/gkx712","article-title":"Chromosome-scale mega-haplotypes enable digital karyotyping of cancer aneuploidy","volume":"45","author":"Bell","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"2026041420021941700_btz503-B3","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1038\/s41467-018-08148-z","article-title":"Multi-platform discovery of haplotype-resolved structural variation in human genomes","volume":"10","author":"Chaisson","year":"2019","journal-title":"Nat. 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