{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T02:36:58Z","timestamp":1784947018929,"version":"3.55.0"},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"24","license":[{"start":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:00:00Z","timestamp":1562025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100015735","name":"HiLife","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015735","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007797","name":"University of Helsinki","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007797","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>Protein structure comparison plays a fundamental role in understanding the evolutionary relationships between proteins. Here, we release a new version of the DaliLite standalone software. The novelties are hierarchical search of the structure database organized into sequence based clusters, and remote access to our knowledge base of structural neighbors. The detection of fold, superfamily and family level similarities by DaliLite and state-of-the-art competitors was benchmarked against a manually curated structural classification.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Database search strategies were evaluated using Fmax with query-specific thresholds. DaliLite and DeepAlign outperformed TM-score based methods at all levels of the benchmark, and DaliLite outperformed DeepAlign at fold level. Hierarchical and knowledge-based searches got close to the performance of systematic pairwise comparison. The knowledge-based search was four times as efficient as the hierarchical search. The knowledge-based search dynamically adjusts the depth of the search, enabling a trade-off between speed and recall.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>http:\/\/ekhidna2.biocenter.helsinki.fi\/dali\/README.v5.html.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btz536","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T11:08:57Z","timestamp":1561720137000},"page":"5326-5327","source":"Crossref","is-referenced-by-count":319,"title":["Benchmarking fold detection by DaliLite v.5"],"prefix":"10.1093","volume":"35","author":[{"given":"Liisa","family":"Holm","sequence":"first","affiliation":[{"name":"Institute of Biotechnology, Helsinki Institute of Life Sciences"},{"name":"University of Helsinki Organismal and Evolutionary Biology Research Program, Faculty of Biosciences, , Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,2]]},"reference":[{"key":"2023013108413909200_btz536-B1","doi-asserted-by":"crossref","first-page":"421.","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"Camacho","year":"2009","journal-title":"BMC Bioinformatics"},{"key":"2023013108413909200_btz536-B2","first-page":"W380","article-title":"mTM-align: a server for fast protein structure database search and multiple protein structure alignment","volume":"46","author":"Dong","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"2023013108413909200_btz536-B3","doi-asserted-by":"crossref","first-page":"D304","DOI":"10.1093\/nar\/gkt1240","article-title":"SCOPe: structural classification of proteins\u2014extended, integrating SCOP and ASTRAL data and classification of new structures","volume":"42","author":"Fox","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"2023013108413909200_btz536-B4","doi-asserted-by":"crossref","first-page":"2780","DOI":"10.1093\/bioinformatics\/btn507","article-title":"Searching protein structure databases with DaliLite v.3","volume":"24","author":"Holm","year":"2008","journal-title":"Bioinformatics"},{"key":"2023013108413909200_btz536-B5","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1093\/bioinformatics\/16.6.566","article-title":"DaliLite workbench for protein structure comparison","volume":"16","author":"Holm","year":"2000","journal-title":"Bioinformatics"},{"key":"2023013108413909200_btz536-B6","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1006\/jmbi.1993.1489","article-title":"Protein structure comparison by alignment of distance matrices","volume":"233","author":"Holm","year":"1993","journal-title":"J. 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