{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T11:58:43Z","timestamp":1770292723932,"version":"3.49.0"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T00:00:00Z","timestamp":1576800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>Open source software such as ImageJ and CellProfiler greatly simplified the quantitative analysis of microscopy images but their applicability is limited by the size, dimensionality and complexity of the images under study. In contrast, software optimized for the needs of specific research projects can overcome these limitations, but they may be harder to find, set up and customize to different needs. Overall, the analysis of large, complex, microscopy images is hence still a critical bottleneck for many Life Scientists. We introduce LOBSTER (Little Objects Segmentation and Tracking Environment), an environment designed to help scientists design and customize image analysis workflows to accurately characterize biological objects from a broad range of fluorescence microscopy images, including large images exceeding workstation main memory. LOBSTER comes with a starting set of over 75 sample image analysis workflows and associated images stemming from state-of-the-art image-based research projects.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>LOBSTER requires MATLAB (version \u2265 2015a), MATLAB Image processing toolbox, and MATLAB statistics and machine learning toolbox. Code source, online tutorials, video demonstrations, documentation and sample images are freely available from: https:\/\/sebastients.github.io.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btz945","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T20:17:10Z","timestamp":1576613830000},"page":"2634-2635","source":"Crossref","is-referenced-by-count":10,"title":["LOBSTER: an environment to design bioimage analysis workflows for large and complex fluorescence microscopy data"],"prefix":"10.1093","volume":"36","author":[{"given":"S\u00e9bastien","family":"Tosi","sequence":"first","affiliation":[{"name":"Institute for Research in Biomedicine\u2013\u2013IRB Barcelona , The Barcelona Institute for Science and Technology\u2013BIST"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00eddia","family":"Bardia","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine\u2013\u2013IRB Barcelona , The Barcelona Institute for Science and Technology\u2013BIST"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jose","family":"Filgueira","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Instituto de Biolog\u00eda Molecular de Barcelona , CSIC, and Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"Calon","sequence":"additional","affiliation":[{"name":"Cancer Research Programme, Hospital del Mar Research Institute (IMIM) , 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Colombelli","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine\u2013\u2013IRB Barcelona , The Barcelona Institute for Science and Technology\u2013BIST"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,12,20]]},"reference":[{"key":"2023013110264071800_btz945-B1","volume-title":"Data Analysis, Machine Learning and Applications. 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