{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T02:21:26Z","timestamp":1771726886896,"version":"3.50.1"},"reference-count":18,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T00:00:00Z","timestamp":1607644800000},"content-version":"vor","delay-in-days":345,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["BJNSF 5182006"],"award-info":[{"award-number":["BJNSF 5182006"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871335, 31660320"],"award-info":[{"award-number":["31871335, 31660320"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the national key research and development program","award":["2018YFA0903702"],"award-info":[{"award-number":["2018YFA0903702"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Essential genes are key elements for organisms to maintain their living. Building databases that store essential genes in the form of homologous clusters, rather than storing them as a singleton, can provide more enlightening information such as the general essentiality of homologous genes in multiple organisms. In 2013, the first database to store prokaryotic essential genes in clusters, CEG (Clusters of Essential Genes), was constructed. Afterward, the amount of available data for essential genes increased by a factor &amp;gt;3 since the last revision. Herein, we updated CEG to version 2, including more prokaryotic essential genes (from 16 gene datasets to 29 gene datasets) and newly added eukaryotic essential genes (nine species), specifically the human essential genes of 12 cancer cell lines. For prokaryotes, information associated with drug targets, such as protein structure, ligand\u2013protein interaction, virulence factor and matched drugs, is also provided. Finally, we provided the service of essential gene prediction for both prokaryotes and eukaryotes. We hope our updated database will benefit more researchers in drug targets and evolutionary genomics.<\/jats:p>\n               <jats:p>Database URL: http:\/\/cefg.uestc.cn\/ceg<\/jats:p>","DOI":"10.1093\/database\/baaa112","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T13:18:51Z","timestamp":1606915131000},"source":"Crossref","is-referenced-by-count":22,"title":["CEG 2.0: an updated database of clusters of essential genes including eukaryotic organisms"],"prefix":"10.1093","volume":"2020","author":[{"given":"Shuo","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China"},{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fa-Zhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan-Nong","family":"Ye","sequence":"additional","affiliation":[{"name":"Bioinformatics and BioMedical Bigdata Mining Laboratory, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Candy-S","family":"Wu","sequence":"additional","affiliation":[{"name":"Thomas Worthington High School, 300 West Granville Road, Worthington, OH 43085, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Xin","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Capital Medical University, Beijing 100069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nini","family":"Rao","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1129-5233","authenticated-orcid":false,"given":"Feng-Biao","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,12,11]]},"reference":[{"key":"2020121110533470500_R1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nrg.2017.74","article-title":"Emerging and evolving concepts in gene essentiality","volume":"19","author":"Rancati","year":"2018","journal-title":"Nat. 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