{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T03:01:17Z","timestamp":1774926077197,"version":"3.50.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"vor","delay-in-days":66,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070659"],"award-info":[{"award-number":["32070659"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Warshel Institute of Computational Biology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ubiquitination is an important post-translational modification, which controls protein turnover by labeling malfunctional and redundant proteins for proteasomal degradation, and also serves intriguing non-proteolytic regulatory functions. E3 ubiquitin ligases, whose substrate specificity determines the recognition of target proteins of ubiquitination, play crucial roles in ubiquitin\u2013proteasome system. UbiNet 2.0 is an updated version of the database UbiNet. It contains 3332 experimentally verified E3\u2013substrate interactions (ESIs) in 54 organisms and rich annotations useful for investigating the regulation of ubiquitination and the substrate specificity of E3 ligases. Based on the accumulated ESIs data, the recognition motifs in substrates for each E3 were also identified and a functional enrichment analysis was conducted on the collected substrates. To facilitate the research on ESIs with different categories of E3 ligases, UbiNet 2.0 performed strictly evidence-based classification of the E3 ligases in the database based on their mechanisms of ubiquitin transfer and substrate specificity. The platform also provides users with an interactive tool that can visualize the ubiquitination network of a group of self-defined proteins, displaying ESIs and protein\u2013protein interactions in a graphical manner. The tool can facilitate the exploration of inner regulatory relationships mediated by ubiquitination among proteins of interest. In summary, UbiNet 2.0 is a user-friendly web-based platform that provides comprehensive as well as updated information about experimentally validated ESIs and a visualized tool for the construction of ubiquitination regulatory networks available at http:\/\/awi.cuhk.edu.cn\/~ubinet\/index.php.<\/jats:p>","DOI":"10.1093\/database\/baab010","type":"journal-article","created":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T17:44:23Z","timestamp":1614275063000},"source":"Crossref","is-referenced-by-count":60,"title":["UbiNet 2.0: a verified, classified, annotated and updated database of E3 ubiquitin ligase\u2013substrate interactions"],"prefix":"10.1093","volume":"2021","author":[{"given":"Zhongyan","family":"Li","sequence":"first","affiliation":[{"name":"School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"}]},{"given":"Siyu","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6158-5207","authenticated-orcid":false,"given":"Jhih-Hua","family":"Jhong","sequence":"additional","affiliation":[{"name":"Warshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6778-7034","authenticated-orcid":false,"given":"Yuxuan","family":"Pang","sequence":"additional","affiliation":[{"name":"School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"}]},{"given":"Kai-Yao","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"},{"name":"Warshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"}]},{"given":"Shangfu","family":"Li","sequence":"additional","affiliation":[{"name":"Warshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8475-7868","authenticated-orcid":false,"given":"Tzong-Yi","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R.China"},{"name":"Warshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P.R. 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