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A workflow to generate hypotheses for the function of these uncharacterized proteins has been developed, based on predicted and experimental information on protein properties, interactions, tissular expression, subcellular localization, conservation in other organisms, as well as phenotypic data in mutant model organisms. This workflow has been applied to seven uncharacterized human proteins (C6orf118, C7orf25, CXorf58, RSRP1, SMLR1, TMEM53 and TMEM232) in the frame of a course-based undergraduate research experience named Functionathon organized at the University of Geneva to teach undergraduate students how to use biological databases and bioinformatics tools and interpret the results. C6orf118, CXorf58 and TMEM232 were proposed to be involved in cilia-related functions; TMEM53 and SMLR1 were proposed to be involved in lipid metabolism and C7orf25 and RSRP1 were proposed to be involved in RNA metabolism and gene expression. Experimental strategies to test these hypotheses were also discussed. The results of this manual data mining study may contribute to the project recently launched by the Human Proteome Organization (HUPO) Human Proteome Project aiming to fill gaps in the functional annotation of human proteins.<\/jats:p>\n               <jats:p>Database URL: http:\/\/www.nextprot.org<\/jats:p>","DOI":"10.1093\/database\/baab046","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T03:12:39Z","timestamp":1626145959000},"source":"Crossref","is-referenced-by-count":11,"title":["Functionathon: a manual data mining workflow to generate functional hypotheses for uncharacterized human proteins and its application by undergraduate students"],"prefix":"10.1093","volume":"2021","author":[{"given":"Paula","family":"Duek","sequence":"first","affiliation":[{"name":"CALIPHO group, SIB Swiss Institute of Bioinformatics"},{"name":"Department of microbiology and molecular medicine, Faculty of medicine, University of Geneva, Geneva, Switzerland"}]},{"given":"Camille","family":"Mary","sequence":"additional","affiliation":[{"name":"Department of microbiology and molecular medicine, Faculty of medicine, University of Geneva, Geneva, Switzerland"}]},{"given":"Monique","family":"Zahn-Zabal","sequence":"additional","affiliation":[{"name":"CALIPHO group, SIB Swiss Institute of Bioinformatics"}]},{"given":"Amos","family":"Bairoch","sequence":"additional","affiliation":[{"name":"CALIPHO group, SIB Swiss Institute of Bioinformatics"},{"name":"Department of microbiology and molecular medicine, Faculty of medicine, University of Geneva, Geneva, Switzerland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9818-3030","authenticated-orcid":false,"given":"Lydie","family":"Lane","sequence":"additional","affiliation":[{"name":"CALIPHO group, SIB Swiss Institute of Bioinformatics"},{"name":"Department of microbiology and molecular medicine, Faculty of medicine, University of Geneva, Geneva, Switzerland"}]}],"member":"286","published-online":{"date-parts":[[2021,7,28]]},"reference":[{"key":"2021072810314280400_R1","first-page":"D328","article-title":"The neXtProt knowledgebase in 2020: data, tools and usability improvements","volume":"48","author":"Zahn-Zabal","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"2021072810314280400_R2","doi-asserted-by":"crossref","first-page":"4042","DOI":"10.1021\/acs.jproteome.8b00383","article-title":"Launching the C-HPP pilot project for functional characterization of identified proteins with no known function","volume":"17","author":"Paik","year":"2018","journal-title":"J. Proteome Res."},{"key":"2021072810314280400_R3","doi-asserted-by":"crossref","first-page":"4211","DOI":"10.1021\/acs.jproteome.8b00537","article-title":"Exploring the uncharacterized human proteome using neXtProt","volume":"17","author":"Duek","year":"2018","journal-title":"J. Proteome Res."},{"key":"2021072810314280400_R4","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.1021\/acs.jproteome.9b00435","article-title":"Worming into the uncharacterized human proteome","volume":"18","author":"Duek","year":"2019","journal-title":"J. Proteome Res."},{"key":"2021072810314280400_R5","doi-asserted-by":"crossref","first-page":"4782","DOI":"10.1021\/acs.jproteome.0c00516","article-title":"The functionally unannotated proteome of human male tissues: a shared resource to uncover new protein functions associated with reproductive biology","volume":"19","author":"Vandenbrouck","year":"2020","journal-title":"J. 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Biol."},{"key":"2021072810314280400_R15","doi-asserted-by":"crossref","first-page":"W296","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: homology modelling of protein structures and complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"2021072810314280400_R16","doi-asserted-by":"crossref","first-page":"W636","DOI":"10.1093\/nar\/gkz268","article-title":"The EMBL-EBI search and sequence analysis tools APIs in2019","volume":"47","author":"Madeira","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2021072810314280400_R17","doi-asserted-by":"crossref","first-page":"D351","DOI":"10.1093\/nar\/gky1100","article-title":"InterPro in 2019: improving coverage, classification and access to protein sequence annotations","volume":"47","author":"Mitchell","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2021072810314280400_R18","doi-asserted-by":"crossref","first-page":"W585","DOI":"10.1093\/nar\/gkm259","article-title":"WoLF PSORT: protein localization predictor","volume":"35","author":"Horton","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"2021072810314280400_R19","doi-asserted-by":"crossref","first-page":"3387","DOI":"10.1093\/bioinformatics\/btx431","article-title":"DeepLoc: prediction of protein subcellular localization using deep learning","volume":"33","author":"Almagro Armenteros","year":"2017","journal-title":"Bioinformatics"},{"key":"2021072810314280400_R20","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. 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Physiol."},{"key":"2021072810314280400_R40","doi-asserted-by":"crossref","first-page":"D899","DOI":"10.1093\/nar\/gkaa1026","article-title":"FlyBase: updates to the Drosophila melanogaster knowledge base","volume":"49","author":"Larkin","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"2021072810314280400_R41","first-page":"D762","article-title":"WormBase: a modern model organism information resource","volume":"48","author":"Harris","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"2021072810314280400_R42","first-page":"416","article-title":"A resource of targeted mutant mouse lines for 5,061 genes","volume":"53","author":"Birling","year":"2019","journal-title":"bioRxiv"},{"key":"2021072810314280400_R43","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. 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