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Herein, to obtain cancer-specific synthetic lethal interactions, we aimed to predict genetic interactions via a pan-cancer analysis from multiple molecular levels using random forest and then develop a user-friendly database. First, based on collected public gene pairs with synthetic lethal interactions, candidate gene pairs were analyzed via integrating multi-omics data, mainly including DNA mutation, copy number variation, methylation and mRNA expression data. Then, integrated features were used to predict cancer-specific synthetic lethal interactions using random forest. Finally, SLOAD (http:\/\/www.tmliang.cn\/SLOAD) was constructed via integrating these findings, which was a user-friendly database for data searching, browsing, downloading and analyzing. These results can provide candidate cancer-specific synthetic lethal interactions, which will contribute to drug designing in cancer treatment that can promote therapy strategies based on the principle of synthetic lethality.<\/jats:p>\n               <jats:p>Database URL http:\/\/www.tmliang.cn\/SLOAD\/<\/jats:p>","DOI":"10.1093\/database\/baac075","type":"journal-article","created":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T15:48:12Z","timestamp":1661615292000},"source":"Crossref","is-referenced-by-count":17,"title":["SLOAD: a comprehensive database of cancer-specific synthetic lethal interactions for precision cancer therapy via multi-omics analysis"],"prefix":"10.1093","volume":"2022","author":[{"given":"Li","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications , No. 9, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"given":"Yuyang","family":"Dou","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications , No. 9, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"given":"Daoliang","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications , No. 9, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"given":"Zibo","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications , No. 9, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"given":"Yangyang","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications , No. 9, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"given":"Lulu","family":"Luo","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology, School of Life Science, Nanjing Normal University , No. 1, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"given":"Yuting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications , No. 9, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Smart Health Big Data Analysis and Location Services Engineering Lab of Jiangsu Province, School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications , No. 9, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6421-0010","authenticated-orcid":false,"given":"Tingming","family":"Liang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology, School of Life Science, Nanjing Normal University , No. 1, Wenyuan Road, Qixia District, Nanjing, Jiangsu 210023, China"}]}],"member":"286","published-online":{"date-parts":[[2022,8,27]]},"reference":[{"key":"2022082715480288500_R1","doi-asserted-by":"publisher","first-page":"1064","DOI":"10.1126\/science.278.5340.1064","article-title":"Integrating genetic approaches into the discovery of anticancer drugs","volume":"278","author":"Hartwell","year":"1997","journal-title":"Science"},{"key":"2022082715480288500_R2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21544-2","article-title":"The tumor therapy landscape of synthetic lethality","volume":"12","author":"Zhang","year":"2021","journal-title":"Nat. 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