{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T19:51:36Z","timestamp":1768679496359,"version":"3.49.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T00:00:00Z","timestamp":1575936000000},"content-version":"vor","delay-in-days":343,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Outstanding Youth Science Foundation of China","award":["31725022"],"award-info":[{"award-number":["31725022"]}]},{"name":"Key Program of Natural Science Foundation of China","award":["31930084"],"award-info":[{"award-number":["31930084"]}]},{"name":"Molecular Enzyme and Engineering International Cooperation Base of South China University of Technology","award":["2017A050503001"],"award-info":[{"award-number":["2017A050503001"]}]},{"name":"Special Program of Guangdong Province for Leader Project in Science and Technology Innovation: Development of New Partial Glycerin Lipase","award":["2015TX01N207"],"award-info":[{"award-number":["2015TX01N207"]}]},{"name":"Marine S&T Fund of Shandong Province","award":["2018SDKJ0302-2"],"award-info":[{"award-number":["2018SDKJ0302-2"]}]},{"name":"National Key R&D Program of China","award":["2018YFD0900503"],"award-info":[{"award-number":["2018YFD0900503"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>There are many unspecific peroxygenases (UPOs) or UPO-like extracellular enzymes secreted by fungal species. These enzymes are considered special in their ways of catalyzing a wide variety of reactions such as epoxidation, peroxygenation and electron oxidations. This enzyme family exhibits diverse functions with thousands of UPOs and UPO-like sequences. These sequences are difficult to analyze without proper management tool and therefore desperately calls for a unified platform that can aide with annotation, classification, navigation and easy sequence retrieval. This prompted us to create an online database called Unspecific Peroxygenase Database (UPObase) (upobase.bioinformaticsreview.com) which currently includes 1948 peroxygenase-encoding protein sequences mined from more than 800 available fungal genomes. It provides information such as classification and motifs about each sequence and has functions such as homology search against UPObase sequence analyses such as multiple sequence alignments and phylogenetic trees. It also provides a new sequence submission facility. The database has been made user-friendly facilitating systematic search and filters. UPObase allows users to search for the sequences by organism name, cluster ID and accession number. Notably, in our previous study, 113 UPOs were classified into five subfamilies (I, II, III, IV and V) and an undetermined group (Pog) which remain established. In this study, using 1948 UPOs in our database, we were able to further identify six novel sub-superfamilies (Pog-a, Pog-b, Pog-c, Pog-d, Pog-e and Pog-f) with signature motifs and two distinct groups in Subfamily I and III, Ia and Ib, IIIa and IIIb, respectively. With the novel UPO-like sequences and classification, UPObase may serve for researchers working in the area of enzyme engineering and related fields.<\/jats:p>","DOI":"10.1093\/database\/baz122","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T20:09:30Z","timestamp":1574453370000},"source":"Crossref","is-referenced-by-count":12,"title":["UPObase: an online database of unspecific peroxygenases"],"prefix":"10.1093","volume":"2019","author":[{"given":"Muniba","family":"Faiza","sequence":"first","affiliation":[{"name":"School of Food Science and Engineering, South China University of Technology, Wushan road, Tianhe district, Guangzhou 510640, Guangdong province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongming","family":"Lan","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering, South China University of Technology, Wushan road, Tianhe district, Guangzhou 510640, Guangdong province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengfeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao) 1 Wenhai road, Aoshanwei, Jimo, Qingdao, Shandong, 266237, China"},{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, No., 135, Xingang Xi road, Guangzhou, 510275, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonghua","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering, South China University of Technology, Wushan road, Tianhe district, Guangzhou 510640, Guangdong province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,12,9]]},"reference":[{"issue":"8","key":"2019121003370970200_ref1","doi-asserted-by":"crossref","first-page":"4575","DOI":"10.1128\/AEM.70.8.4575-4581.2004","article-title":"Novel haloperoxidase from the agaric basidiomycete Agrocybe aegerita oxidizes aryl alcohols and aldehydes.","volume":"70","author":"Ullrich","year":"2004","journal-title":"Appl Environ Microbiol [Internet]."},{"key":"2019121003370970200_ref2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.abb.2011.08.001","article-title":"Regioselective oxygenation of fatty acids, fatty alcohols and other aliphatic compounds by a basidiomycete heme-thiolate peroxidase.","volume":"514","author":"Guti\u00e9rrez","year":"2011","journal-title":"Arch Biochem Biophys [Internet]."},{"key":"2019121003370970200_ref3","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.cbpa.2014.01.015","article-title":"Oxidations catalyzed by fungal peroxygenases","volume":"19","author":"Hofrichter","year":"2014","journal-title":"Curr. 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