{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T01:44:59Z","timestamp":1764985499187,"version":"3.46.0"},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["www.embopress.org"],"crossmark-restriction":false},"short-container-title":["EMBO Reports"],"published-print":{"date-parts":[[2000,9]]},"abstract":"\n The\n D<\/jats:sc>\n \u2010glucuronyltransferase and\n N<\/jats:italic>\n \u2010acetyl\u2010\n D<\/jats:sc>\n \u2010glucosaminyltransferase reactions in heparan sulfate biosynthesis have been associated with two genes,\n EXT1<\/jats:italic>\n and\n EXT2<\/jats:italic>\n , which are also implicated in the inherited bone disorder, multiple exostoses. Since the cell systems used to express recombinant EXT proteins synthesize endogenous heparan sulfate, and the EXT proteins tend to associate, it has not been possible to define the functional roles of the individual protein species. We therefore expressed EXT1 and EXT2 in yeast, which does not synthesize heparan sulfate. The recombinant EXT1 and EXT2 were both found to catalyze both glycosyltransferase reactions\n in vitro<\/jats:italic>\n . Coexpression of the two proteins, but not mixing of separately expressed recombinant EXT1 and EXT2, yields hetero\u2010oligomeric complexes in yeast and mammalian cells, with augmented glycosyltransferase activities. This stimulation does not depend on the membrane\u2010bound state of the proteins.\n <\/jats:p>","DOI":"10.1093\/embo-reports\/kvd045","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T18:48:15Z","timestamp":1027709295000},"page":"282-286","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":148,"title":["The EXT1\/EXT2 tumor suppressors: catalytic activities and role in heparan sulfate biosynthesis"],"prefix":"10.1038","volume":"1","author":[{"given":"Claire","family":"Senay","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, University of Uppsala, The Biomedical Center, Box 582 S\u2010751 23 Uppsala Sweden"}]},{"given":"Thomas","family":"Lind","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, University of Uppsala, The Biomedical Center, Box 582 S\u2010751 23 Uppsala Sweden"}]},{"given":"Kumi","family":"Muguruma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kobe Pharmaceutical University Kobe Japan"}]},{"given":"Yuko","family":"Tone","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kobe Pharmaceutical University Kobe Japan"}]},{"given":"Hiroshi","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kobe Pharmaceutical University Kobe Japan"}]},{"given":"Kazuyuki","family":"Sugahara","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kobe Pharmaceutical University Kobe Japan"}]},{"given":"Kerstin","family":"Lidholt","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, University of Uppsala, The Biomedical Center, Box 582 S\u2010751 23 Uppsala Sweden"}]},{"given":"Ulf","family":"Lindahl","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, University of Uppsala, The Biomedical Center, Box 582 S\u2010751 23 Uppsala Sweden"}]},{"given":"Marion","family":"Kusche\u2010Gullberg","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, University of Uppsala, The Biomedical Center, Box 582 S\u2010751 23 Uppsala Sweden"}]}],"member":"297","published-online":{"date-parts":[[2000,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1095-137"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(98)00126-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1385\/0-89603-421-6:41","article-title":"Small vectors for expression based on dominant drug resistance with direct multicopy selection","volume":"103","author":"Higgins D.R.","year":"1998","journal-title":"Methods Mol. 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