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We employed Cre\/\n                    <jats:italic>loxP<\/jats:italic>\n                    \u2010mediated gene targeting to disrupt the ubiquitously expressed N\u2010WASP in the mouse germline, which led to embryonic lethality. To elucidate the role of N\u2010WASP at the cellular level, we immortalized embryonic fibroblasts and selected various N\u2010WASP\u2010defective cell lines. These fibroblasts showed no apparent morphological alterations and were highly responsive to the induction of filopodia, but failed to support the motility of\n                    <jats:italic>Shigella flexneri<\/jats:italic>\n                    . In addition, enteropathogenic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    were incapable of inducing the formation of actin pedestals in N\u2010WASP\u2010defective cells. Our results prove the essential role of this protein for actin cytoskeletal changes induced by these bacterial pathogens\n                    <jats:italic>in vivo<\/jats:italic>\n                    and in addition show for the first time that N\u2010WASP is dispensible for filopodia formation.\n                  <\/jats:p>","DOI":"10.1093\/embo-reports\/kve197","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T18:49:38Z","timestamp":1027709378000},"page":"850-857","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":227,"title":["Actin pedestal formation by enteropathogenic\n                    <i>Escherichia coli<\/i>\n                    and intracellular motility of\n                    <i>Shigella flexneri<\/i>\n                    are abolished in N\u2010WASP\u2010defective cells"],"prefix":"10.1038","volume":"2","author":[{"given":"Silvia","family":"Lommel","sequence":"first","affiliation":[{"name":"Institute for Genetics, University of K\u00f6ln  Weyertal 121 50931 K\u00f6ln Germany"},{"name":"Department of Cell Biology, Gesellschaft f\u00fcr Biotechnologische Forschung (GBF)  Mascheroder Weg 1 38124 Braunschweig Germany"}]},{"given":"Stefanie","family":"Benesch","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Gesellschaft f\u00fcr Biotechnologische Forschung (GBF)  Mascheroder Weg 1 38124 Braunschweig Germany"}]},{"given":"Klemens","family":"Rottner","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Gesellschaft f\u00fcr Biotechnologische Forschung (GBF)  Mascheroder Weg 1 38124 Braunschweig Germany"}]},{"given":"Thomas","family":"Franz","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of Bonn  Nussallee 10 53115 Bonn Germany"}]},{"given":"J\u00fcrgen","family":"Wehland","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Gesellschaft f\u00fcr Biotechnologische Forschung (GBF)  Mascheroder Weg 1 38124 Braunschweig Germany"}]},{"given":"Ralf","family":"K\u00fchn","sequence":"additional","affiliation":[{"name":"Present address: Artemis Pharmaceuticals GmbH  Neurather Ring 1 51063 K\u00f6ln 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