{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T07:01:05Z","timestamp":1774681265789,"version":"3.50.1"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ningxia Key Research and Development Program","award":["2016BZ05"],"award-info":[{"award-number":["2016BZ05"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31701120"],"award-info":[{"award-number":["31701120"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Lycium barbarum, a member of the Solanaceae family, is an important eudicot with applications in both food and medicine. L. barbarum pectin polysaccharides (LBPPs) are key bioactive compounds of L. barbarum, notable for being among the few polysaccharides with both biocompatibility and biomedical activity. Although studies have analyzed the functional properties of LBPPs, the mechanisms\u00a0underlying their biosynthesis and transport by key enzymes remain poorly understood. In this study, we assembled a 2.18-Gb reference genome of L. barbarum, reconstructed the first complete biosynthesis pathway of LBPPs, and elucidated the sugar transport system. We also characterized the important genes responsible for backbone extension, sidechain synthesis, and modification of LBPPs. Furthermore, we characterized the long non-coding RNAs (lncRNAs) associated with polysaccharide metabolism. We identified a specific rhamnogalacturonan I (RG-I) rhamnosyltransferase, RRT3020, which enhances RG-I biosynthesis within LBPPs. These newly identified enzymes and pivotal genes endow L. barbarum with unique pectin biosynthesis capabilities, distinguishing it from other Solanaceae species. Our findings thus provide a foundation for evolutionary studies and molecular breeding to expand\u00a0the diverse applications of L. barbarum.<\/jats:p>","DOI":"10.1093\/gpbjnl\/qzae079","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T14:58:33Z","timestamp":1730732313000},"source":"Crossref","is-referenced-by-count":2,"title":["Enzyme Repertoires and Genomic Insights into <i>Lycium barbarum<\/i> Pectin Polysaccharide Biosynthesis"],"prefix":"10.1093","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8556-4635","authenticated-orcid":false,"given":"Haiyan","family":"Yue","sequence":"first","affiliation":[{"name":"State Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"CAS Key Laboratory of RNA Biology, Center for Big Data Research in Health, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9484-3353","authenticated-orcid":false,"given":"Yiheng","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049,","place":["China"]},{"name":"German Cancer Research Center (DKFZ) , 69120 Heidelberg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8926-8710","authenticated-orcid":false,"given":"Aixuan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"College of Life 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Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8848-5248","authenticated-orcid":false,"given":"Yiming","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6645-2434","authenticated-orcid":false,"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5550-9889","authenticated-orcid":false,"given":"Jianjun","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 100049,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6046-8420","authenticated-orcid":false,"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing University of Chinese Medicine , Beijing 100029,","place":["China"]},{"name":"Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences , Beijing 100190,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7294-0865","authenticated-orcid":false,"given":"Shunmin","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]},{"name":"CAS Key Laboratory of RNA Biology, Center for Big Data Research in Health, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1008-1062","authenticated-orcid":false,"given":"Chang","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, University of Chinese Academy of Sciences , Beijing 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