{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T14:04:43Z","timestamp":1781618683462,"version":"3.54.5"},"reference-count":98,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T00:00:00Z","timestamp":1740182400000},"content-version":"vor","delay-in-days":21,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mass spectrometry-based single-cell proteomics (MS-SCP) is attracting tremendous attention because it is now technically feasible to quantify thousands of proteins in minute samples. Since protein amplification is still not possible, technological improvements in MS-SCP focus on minimizing sample loss while increasing throughput, resolution, and sensitivity, as well as achieving measurement depth, accuracy, and stability comparable to bulk samples. Major advances in MS-SCP have facilitated its application in biological and even medical research. Here, we review the key advancements in MS-SCP technology and discuss the strategies of the typical proteomics workflow to improve MS-SCP analysis from single-cell isolation, sample preparation, and liquid chromatography separation to MS data acquisition and analysis. The review will provide an overall understanding of the development and applications of MS-SCP and inspire more novel ideas regarding the innovation of MS-SCP technology.<\/jats:p>","DOI":"10.1093\/gpbjnl\/qzaf012","type":"journal-article","created":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T09:17:15Z","timestamp":1740215835000},"source":"Crossref","is-referenced-by-count":9,"title":["Mass Spectrometry-based Solutions for Single-cell Proteomics"],"prefix":"10.1093","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4026-963X","authenticated-orcid":false,"given":"Siqi","family":"Li","sequence":"first","affiliation":[{"name":"HIM-BGI Omics Center, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences , Hangzhou 310022,","place":["China"]},{"name":"BGI-Shenzhen , Shenzhen 518083,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7072-0052","authenticated-orcid":false,"given":"Shuwei","family":"Li","sequence":"additional","affiliation":[{"name":"Nanjing Apollomics Biotech Inc ., Nanjing 210033,","place":["China"]},{"name":"China Pharmaceutical University , Nanjing 210009,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9744-3681","authenticated-orcid":false,"given":"Siqi","family":"Liu","sequence":"additional","affiliation":[{"name":"HIM-BGI Omics Center, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences , Hangzhou 310022,","place":["China"]},{"name":"BGI-Shenzhen , Shenzhen 518083,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-8625","authenticated-orcid":false,"given":"Yan","family":"Ren","sequence":"additional","affiliation":[{"name":"HIM-BGI Omics Center, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences , Hangzhou 310022,","place":["China"]},{"name":"Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine , Shanghai 201203,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,2,22]]},"reference":[{"key":"2025072404365717200_qzaf012-B1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/B978-0-12-814919-5.00014-2","volume-title":"Single-cell omics","author":"Minakshi","year":"2019"},{"key":"2025072404365717200_qzaf012-B2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/nmeth.1315","article-title":"mRNA-seq 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