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However, only a minority of patients respond to treatment due to the stochastic heterogeneity of tumor microenvironment (TME). Recent advances in single-cell RNA-seq technologies enabled comprehensive characterization of the immune system heterogeneity in tumors but posed computational challenges on integrating and utilizing the massive published datasets to inform immunotherapy. Here, we present Tumor Immune Single Cell Hub (TISCH, http:\/\/tisch.comp-genomics.org), a large-scale curated database that integrates single-cell transcriptomic profiles of nearly 2 million cells from 76 high-quality tumor datasets across 27 cancer types. All the data were uniformly processed with a standardized workflow, including quality control, batch effect removal, clustering, cell-type annotation, malignant cell classification, differential expression analysis and functional enrichment analysis. TISCH provides interactive gene expression visualization across multiple datasets at the single-cell level or cluster level, allowing systematic comparison between different cell-types, patients, tissue origins, treatment and response groups, and even different cancer-types. In summary, TISCH provides a user-friendly interface for systematically visualizing, searching and downloading gene expression atlas in the TME from multiple cancer types, enabling fast, flexible and comprehensive exploration of the TME.<\/jats:p>","DOI":"10.1093\/nar\/gkaa1020","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T07:11:02Z","timestamp":1602832262000},"page":"D1420-D1430","source":"Crossref","is-referenced-by-count":881,"title":["TISCH: a comprehensive web resource enabling interactive single-cell transcriptome visualization of tumor microenvironment"],"prefix":"10.1093","volume":"49","author":[{"given":"Dongqing","family":"Sun","sequence":"first","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Ya","family":"Han","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Xin","family":"Dong","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Jun","family":"Ge","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Rongbin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Xiaoying","family":"Shi","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Binbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Ziyi","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Pengfei","family":"Ren","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]},{"given":"Liangdong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433, China"}]},{"given":"Yilv","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433, China"}]},{"given":"Peng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433, China"}]},{"given":"Fan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Clinical Translational Research Center, Shanghai Pulmonary Hospital, School of Life Science, Tongji University,\u00a0Shanghai 200433, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7940-8196","authenticated-orcid":false,"given":"Taiwen","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management, West China Hospital of Stomatology, Sichuan University,\u00a0Chengdu, Sichuan 610041, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7573-3768","authenticated-orcid":false,"given":"Chenfei","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Putuo District People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200060, China"}]}],"member":"286","published-online":{"date-parts":[[2020,11,12]]},"reference":[{"key":"2021010313130734100_B1","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1016\/S0140-6736(16)31679-8","article-title":"Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990\u20132015: a systematic analysis for the Global Burden of Disease Study 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