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It contains several thermodynamic parameters such as melting temperature, free energy obtained with thermal and denaturant denaturation, enthalpy change and heat capacity change along with experimental methods and conditions, sequence, structure and literature information. Besides, the current version of the database includes about 120 000 thermodynamic data obtained for different organisms and cell lines, which are determined by recent high throughput proteomics techniques using whole-cell approaches. In addition, we provided a graphical interface for visualization of mutations at sequence and structure levels. ProThermDB is cross-linked with other relevant databases, PDB, UniProt, PubMed etc. It is freely available at https:\/\/web.iitm.ac.in\/bioinfo2\/prothermdb\/index.html without any login requirements. It is implemented in Python, HTML and JavaScript, and supports the latest versions of major browsers, such as Firefox, Chrome and Safari.<\/jats:p>","DOI":"10.1093\/nar\/gkaa1035","type":"journal-article","created":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T08:10:39Z","timestamp":1603699839000},"page":"D420-D424","source":"Crossref","is-referenced-by-count":209,"title":["ProThermDB: thermodynamic database for proteins and mutants revisited after 15 years"],"prefix":"10.1093","volume":"49","author":[{"given":"Rahul","family":"Nikam","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of BioSciences, Indian Institute of Technology Madras, Chennai 600 036, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Kulandaisamy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of BioSciences, Indian Institute of Technology Madras, Chennai 600 036, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Harini","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of BioSciences, Indian Institute of Technology Madras, Chennai 600 036, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of BioSciences, Indian Institute of Technology Madras, Chennai 600 036, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1776-4096","authenticated-orcid":false,"given":"M Michael","family":"Gromiha","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of BioSciences, Indian Institute of Technology Madras, Chennai 600 036, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,11,16]]},"reference":[{"key":"2021010313114734900_B1","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-56462-8","volume-title":"Protein Stability and Folding, Supplement 1: A Collection of Thermodynamic Data","author":"Pfeil","year":"2001"},{"key":"2021010313114734900_B2","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1021\/bi9817820","article-title":"Effects of core mutations on the folding of a \u03b2-sheet protein: implications for backbone organization in the I-state","volume":"38","author":"Lorch","year":"1999","journal-title":"Biochemistry"},{"key":"2021010313114734900_B3","doi-asserted-by":"crossref","first-page":"3480","DOI":"10.1021\/bi9923510","article-title":"Effects of mutations on the thermodynamics of a protein folding reaction: implications for the mechanism of formation of the intermediate and transition states","volume":"39","author":"Lorch","year":"2000","journal-title":"Biochemistry"},{"key":"2021010313114734900_B4","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.arcmed.2006.01.006","article-title":"Catalytic inactivation of human phospholipase D2 by a naturally occurring Gly901Asp mutation","volume":"37","author":"Yamada","year":"2006","journal-title":"Arch. 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