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It can also be used for searching latent allosteric sites and for computationally designing allosteric effectors for these sites with required agonist\/antagonist activity. The server is based on the implementation of the Structure-Based Statistical Mechanical Model of Allostery (SBSMMA), which allows one to evaluate the allosteric free energy as a result of the perturbation at per-residue resolution. The Allosteric Signaling Map (ASM) providing a comprehensive residue-by-residue allosteric control over the protein activity can be obtained for any structure of interest. The Allosteric Probing Map (APM), in turn, allows one to perform the fragment-based-like computational design experiment aimed at finding leads for potential allosteric effectors. The server can be instrumental in elucidating of allosteric mechanisms and actions of allosteric mutations, and in the efforts on design of new elements of allosteric control. The server is freely available at: http:\/\/allosigma.bii.a-star.edu.sg<\/jats:p>","DOI":"10.1093\/nar\/gkaa338","type":"journal-article","created":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T19:09:43Z","timestamp":1587582583000},"page":"W116-W124","source":"Crossref","is-referenced-by-count":88,"title":["AlloSigMA 2: paving the way to designing allosteric effectors and to exploring allosteric effects of mutations"],"prefix":"10.1093","volume":"48","author":[{"given":"Zhen Wah","family":"Tan","sequence":"first","affiliation":[{"name":"Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), 30 Biopolis Street, #07-01, Matrix, 138671, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Guarnera","sequence":"first","affiliation":[{"name":"Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), 30 Biopolis Street, #07-01, Matrix, 138671, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Ven","family":"Tee","sequence":"first","affiliation":[{"name":"Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), 30 Biopolis Street, #07-01, Matrix, 138671, Singapore"},{"name":"Department of Biological Sciences (DBS), National University of Singapore (NUS), 8 Medical Drive, 117579, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3315-8483","authenticated-orcid":false,"given":"Igor N","family":"Berezovsky","sequence":"first","affiliation":[{"name":"Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), 30 Biopolis Street, #07-01, Matrix, 138671, Singapore"},{"name":"Department of Biological Sciences (DBS), National University of Singapore (NUS), 8 Medical Drive, 117579, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,5,11]]},"reference":[{"key":"2020062614045732900_B1","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/j.bbapap.2013.01.024","article-title":"Thermodynamics of allostery paves a way to allosteric drugs","volume":"1834","author":"Berezovsky","year":"2013","journal-title":"Biochim. 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