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Since its first release in 2016, miRNet has been accessed by &amp;gt;20 000 researchers worldwide, with \u223c100 users on a daily basis. While version 1.0 was focused primarily on miRNA-target gene interactions, it has become clear that in order to obtain a global view of miRNA functions, it is necessary to bring other important players into the context during analysis. Driven by this concept, in miRNet version 2.0, we have (i) added support for transcription factors (TFs) and single nucleotide polymorphisms (SNPs) that affect miRNAs, miRNA-binding sites or target genes, whilst also greatly increased (&amp;gt;5-fold) the underlying knowledgebases of miRNAs, ncRNAs and disease associations; (ii) implemented new functions to allow creation and visual exploration of multipartite networks, with enhanced support for in situ functional analysis and (iii) revamped the web interface, optimized the workflow, and introduced microservices and web application programming interface (API) to sustain high-performance, real-time data analysis. The underlying R package is also released in tandem with version 2.0 to allow more flexible data analysis for R programmers. The miRNet 2.0 website is freely available at https:\/\/www.mirnet.ca.<\/jats:p>","DOI":"10.1093\/nar\/gkaa467","type":"journal-article","created":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T03:22:33Z","timestamp":1590117753000},"page":"W244-W251","source":"Crossref","is-referenced-by-count":824,"title":["miRNet 2.0: network-based visual analytics for miRNA functional analysis and systems biology"],"prefix":"10.1093","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0966-7923","authenticated-orcid":false,"given":"Le","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Human Genetics, McGill University, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyan","family":"Zhou","sequence":"first","affiliation":[{"name":"Institute of Parasitology, McGill University, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Othman","family":"Soufan","sequence":"first","affiliation":[{"name":"Institute of Parasitology, McGill University, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2040-2624","authenticated-orcid":false,"given":"Jianguo","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Human Genetics, McGill University, Montreal, Quebec, Canada"},{"name":"Institute of Parasitology, McGill University, Montreal, Quebec, Canada"},{"name":"Department of Animal Science, McGill University, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,6,2]]},"reference":[{"key":"2020062614042343900_B1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S0092-8674(02)00655-4","article-title":"A unified theory of gene expression","volume":"108","author":"Orphanides","year":"2002","journal-title":"Cell"},{"key":"2020062614042343900_B2","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1101\/gad.1937010","article-title":"MicroRNAs and gene regulatory networks: managing the impact of noise in biological systems","volume":"24","author":"Herranz","year":"2010","journal-title":"Genes Dev."},{"key":"2020062614042343900_B3","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s00438-013-0734-z","article-title":"miRNA-transcription factor interactions: a combinatorial regulation of gene expression","volume":"288","author":"Arora","year":"2013","journal-title":"Mol. 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