{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:50:32Z","timestamp":1770223832323,"version":"3.49.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T00:00:00Z","timestamp":1601683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001797","name":"Pharmaceutical Research and Manufacturers of America Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001797","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Center of Genetic Medicine Research"},{"name":"W.T. Gill Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>High-throughput genetic screening based on CRISPR\/Cas9 or RNA-interference (RNAi) enables the exploration of genes associated with the phenotype of interest on a large scale. The rapid accumulation of public available genetic screening data provides a wealth of knowledge about genotype-to-phenotype relationships and a valuable resource for the systematic analysis of gene functions. Here we present CRISP-view, a comprehensive database of CRISPR\/Cas9 and RNAi screening datasets that span multiple phenotypes, including in vitro and in vivo cell proliferation and viability, response to cancer immunotherapy, virus response, protein expression, etc. By 22\u00a0September\u00a02020, CRISP-view has collected 10 321\u00a0human samples and 825\u00a0mouse samples from 167\u00a0papers. All the datasets have been curated, annotated, and processed by a standard MAGeCK-VISPR analysis pipeline with quality control (QC) metrics. We also developed a user-friendly webserver to visualize, explore, and search these datasets. The webserver is freely available at http:\/\/crispview.weililab.org.<\/jats:p>","DOI":"10.1093\/nar\/gkaa809","type":"journal-article","created":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T12:36:32Z","timestamp":1601469392000},"page":"D848-D854","source":"Crossref","is-referenced-by-count":20,"title":["CRISP-view: a database of functional genetic screens spanning multiple phenotypes"],"prefix":"10.1093","volume":"49","author":[{"given":"Yingbo","family":"Cui","sequence":"first","affiliation":[{"name":"Sanyi Road, Changsha, Hunan Province, People's Republic of China"}]},{"given":"Xiaolong","family":"Cheng","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"}]},{"given":"Qing","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"}]},{"given":"Bicna","family":"Song","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"}]},{"given":"Anthony","family":"Chiu","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"},{"name":"School of Medicine and Health Sciences, George Washington University, 2300 I Street NW, Washington, DC 20037, USA"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"},{"name":"Department of Biochemistry and Molecular Biology, George Washington University, 2300 I Street NW, Washington, DC 20037, USA"}]},{"given":"Tyson","family":"Dawson","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"},{"name":"Institute for Biomedical Sciences, George Washington University, 2300 I Street NW, Washington, DC 20037, USA"},{"name":"Computational Biology Institute, Milken Institute School of Public Health, George Washington University, 45085 University Drive, Ashburn, VA 20148, USA"}]},{"given":"Lumen","family":"Chao","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"}]},{"given":"Wubing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Data Sciences, Dana-Farber Cancer Institute and Harvard T.H. Chan School of Public Health. 450 Brookline Ave., Boston MA 02215, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5374-7314","authenticated-orcid":false,"given":"Dian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Data Sciences, Dana-Farber Cancer Institute and Harvard T.H. Chan School of Public Health. 450 Brookline Ave., Boston MA 02215, USA"}]},{"given":"Zexiang","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Data Sciences, Dana-Farber Cancer Institute and Harvard T.H. Chan School of Public Health. 450 Brookline Ave., Boston MA 02215, USA"}]},{"given":"Jijun","family":"Yu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Therapeutic Gene Engineering Antibody. Beijing, People's Republic of China"}]},{"given":"Zexu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life and Health Sciences, Northeastern University. 110819 Shenyang, People's Republic of China"}]},{"given":"Teng","family":"Fei","sequence":"additional","affiliation":[{"name":"College of Life and Health Sciences, Northeastern University. 110819 Shenyang, People's Republic of China"}]},{"given":"Shaoliang","family":"Peng","sequence":"additional","affiliation":[{"name":"Lushan South Road, Changsha, Hunan Province, People's Republic of China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2163-7903","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA"},{"name":"Department of Genomics and Precision Medicine, George Washington University. 111 Michigan Ave NW, Washington, DC 20010, USA"}]}],"member":"286","published-online":{"date-parts":[[2020,10,3]]},"reference":[{"key":"2021010313111619700_B1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1126\/science.1246981","article-title":"Genetic screens in human cells using the CRISPR-Cas9 system","volume":"343","author":"Wang","year":"2014","journal-title":"Science"},{"key":"2021010313111619700_B2","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.cell.2015.02.038","article-title":"Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis","volume":"160","author":"Chen","year":"2015","journal-title":"Cell"},{"key":"2021010313111619700_B3","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nbt.2501","article-title":"Efficient genome editing in zebrafish using a CRISPR-Cas system","volume":"31","author":"Hwang","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"2021010313111619700_B4","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/nature13166","article-title":"High-throughput screening of a CRISPR\/Cas9 library for functional genomics in human cells","volume":"509","author":"Zhou","year":"2014","journal-title":"Nature"},{"key":"2021010313111619700_B5","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nbt.2800","article-title":"Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library","volume":"32","author":"Koike-Yusa","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"2021010313111619700_B6","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1126\/science.1246981","article-title":"Genetic screens in human cells using the CRISPR-Cas9 system","volume":"343","author":"Wang","year":"2014","journal-title":"Science"},{"key":"2021010313111619700_B7","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-scale CRISPR-Cas9 knockout screening in human cells","volume":"343","author":"Shalem","year":"2014","journal-title":"Science"},{"key":"2021010313111619700_B8","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/nrg1248","article-title":"Systematic genome-wide screens of gene function","volume":"5","author":"Carpenter","year":"2004","journal-title":"Nat. Rev. Genet."},{"key":"2021010313111619700_B9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1038\/418244a","article-title":"RNA interference","volume":"418","author":"Hannon","year":"2002","journal-title":"Nature"},{"key":"2021010313111619700_B10","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/nmeth1006-777","article-title":"Minimizing the risk of reporting false positives in large-scale RNAi screens","volume":"3","author":"Echeverri","year":"2006","journal-title":"Nat. Methods"},{"key":"2021010313111619700_B11","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nrg1836","article-title":"High-throughput RNAi screening in cultured cells: a user's guide","volume":"7","author":"Echeverri","year":"2006","journal-title":"Nat. Rev. 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Cell"},{"key":"2021010313111619700_B20","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1038\/s41467-020-14811-1","article-title":"Haploid genetic screens identify SPRING\/C12ORF49 as a determinant of SREBP signaling and cholesterol metabolism","volume":"11","author":"Loregger","year":"2020","journal-title":"Nat. Commun."},{"key":"2021010313111619700_B21","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/nature15521","article-title":"BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis","volume":"527","author":"Canver","year":"2015","journal-title":"Nature"},{"key":"2021010313111619700_B22","doi-asserted-by":"crossref","first-page":"25186","DOI":"10.1073\/pnas.1908155116","article-title":"Deciphering essential cistromes using genome-wide CRISPR screens","volume":"116","author":"Fei","year":"2019","journal-title":"Proc. Natl. Acad. Sci. 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Natl. Acad. Sci. U.S.A."},{"key":"2021010313111619700_B35","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-scale CRISPR-Cas9 knockout screening in human cells","volume":"343","author":"Shalem","year":"2014","journal-title":"Science"}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/49\/D1\/D848\/35364019\/gkaa809.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/49\/D1\/D848\/35364019\/gkaa809.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,3]],"date-time":"2021-01-03T18:11:54Z","timestamp":1609697514000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article\/49\/D1\/D848\/5917659"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,3]]},"references-count":35,"journal-issue":{"issue":"D1","published-online":{"date-parts":[[2020,10,3]]},"published-print":{"date-parts":[[2021,1,8]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkaa809","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,1,8]]},"published":{"date-parts":[[2020,10,3]]}}}