{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:24:42Z","timestamp":1791523482082,"version":"4.3.4"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U24DK116204"],"award-info":[{"award-number":["U24DK116204"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Kinases form the backbone of numerous cell signaling pathways, with their dysfunction similarly implicated in multiple pathologies. Further facilitated by their druggability, kinases are a major focus of therapeutic development efforts in diseases such as cancer, infectious disease and autoimmune disorders. While their importance is clear, the role or biological function of nearly one-third of kinases is largely unknown. Here, we describe a data resource, the Dark Kinase Knowledgebase (DKK; https:\/\/darkkinome.org), that is specifically focused on providing data and reagents for these understudied kinases to the broader research community. Supported through NIH\u2019s Illuminating the Druggable Genome (IDG) Program, the DKK is focused on data and knowledge generation for 162 poorly studied or \u2018dark\u2019 kinases. Types of data provided through the DKK include parallel reaction monitoring (PRM) peptides for quantitative proteomics, protein interactions, NanoBRET reagents, and kinase-specific compounds. Higher-level data is similarly being generated and consolidated such as tissue gene expression profiles and, longer-term, functional relationships derived through perturbation studies. Associated web tools that help investigators interrogate both internal and external data are also provided through the site. As an evolving resource, the DKK seeks to continually support and enhance knowledge on these potentially high-impact druggable targets.<\/jats:p>","DOI":"10.1093\/nar\/gkaa853","type":"journal-article","created":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T11:12:53Z","timestamp":1601118773000},"page":"D529-D535","source":"Crossref","is-referenced-by-count":140,"title":["The Dark Kinase Knowledgebase: an online compendium of knowledge and experimental results of understudied kinases"],"prefix":"10.1093","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4798-3273","authenticated-orcid":false,"given":"Matthew E","family":"Berginski","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nienke","family":"Moret","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Department of Systems Biology, Harvard Program in Therapeutic Science, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchang","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Department of Systems Biology, Harvard Program in Therapeutic Science, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Goldfarb","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, Washington University in St.\u00a0Louis, St. Louis, MO 63110, USA"},{"name":"Institute for Informatics, Washington University in St. Louis, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter\u00a0K","family":"Sorger","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Department of Systems Biology, Harvard Program in Therapeutic Science, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8251-4552","authenticated-orcid":false,"given":"Shawn M","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"},{"name":"Joint Department of Biomedical Engineering at the University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,20]]},"reference":[{"key":"2021010313132410800_B1","doi-asserted-by":"crossref","DOI":"10.1101\/2020.04.02.022277","article-title":"Exploring the understudied human kinome for research and therapeutic opportunities","author":"Moret","year":"2020"},{"key":"2021010313132410800_B2","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s12859-016-1433-7","article-title":"KinMap: a web-based tool for interactive navigation through human kinome data","volume":"18","author":"Eid","year":"2017","journal-title":"BMC Bioinformatics"},{"key":"2021010313132410800_B3","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1126\/science.1075762","article-title":"The protein kinase complement of the human genome","volume":"298","author":"Manning","year":"2002","journal-title":"Science"},{"key":"2021010313132410800_B4","doi-asserted-by":"crossref","first-page":"eaav3810","DOI":"10.1126\/scisignal.aav3810","article-title":"Tracing the origin and evolution of pseudokinases across the tree of life","volume":"12","author":"Kwon","year":"2019","journal-title":"Sci. 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