{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T01:17:30Z","timestamp":1793063850459,"version":"4.5.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T00:00:00Z","timestamp":1636329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NaturalAntibody"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Nanobodies, a subclass of antibodies found in camelids, are versatile molecular binding scaffolds composed of a single polypeptide chain. The small size of nanobodies bestows multiple therapeutic advantages (stability, tumor penetration) with the first therapeutic approval in 2018 cementing the clinical viability of this format. Structured data and sequence information of nanobodies will enable the accelerated clinical development of nanobody-based therapeutics. Though the nanobody sequence and structure data are deposited in the public domain at an accelerating pace, the heterogeneity of sources and lack of standardization hampers reliable harvesting of nanobody information. We address this issue by creating the Integrated Database of Nanobodies for Immunoinformatics (INDI, http:\/\/naturalantibody.com\/nanobodies). INDI collates nanobodies from all the major public outlets of biological sequences: patents, GenBank, next-generation sequencing repositories, structures and scientific publications. We equip INDI with powerful nanobody-specific sequence and text search facilitating access to &amp;gt;11 million nanobody sequences. INDI should facilitate development of novel nanobody-specific computational protocols helping to deliver on the therapeutic promise of this drug format.<\/jats:p>","DOI":"10.1093\/nar\/gkab1021","type":"journal-article","created":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T19:02:41Z","timestamp":1634583761000},"page":"D1273-D1281","source":"Crossref","is-referenced-by-count":76,"title":["INDI\u2014integrated nanobody database for immunoinformatics"],"prefix":"10.1093","volume":"50","author":[{"given":"Piotr","family":"Deszy\u0144ski","sequence":"first","affiliation":[{"name":"NaturalAntibody, Al. Piast\u00f3w 22, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"M\u0142okosiewicz","sequence":"additional","affiliation":[{"name":"NaturalAntibody, Al. Piast\u00f3w 22, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Volanakis","sequence":"additional","affiliation":[{"name":"Harvard Medical School, 240 Longwood Ave, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Jaszczyszyn","sequence":"additional","affiliation":[{"name":"NaturalAntibody, Al. Piast\u00f3w 22, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Castellana","sequence":"additional","affiliation":[{"name":"Abterra Biosciences Inc. 3030 Bunker Hill Street Suite 218, San Diego, CA 92109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Bonissone","sequence":"additional","affiliation":[{"name":"Abterra Biosciences Inc. 3030 Bunker Hill Street Suite 218, San Diego, CA 92109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajkumar","family":"Ganesan","sequence":"additional","affiliation":[{"name":"Alector, South San Francisco, CA 94080, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0697-5522","authenticated-orcid":false,"given":"Konrad","family":"Krawczyk","sequence":"additional","affiliation":[{"name":"NaturalAntibody, Al. Piast\u00f3w 22, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,11,8]]},"reference":[{"key":"2022010507305258700_B1","volume-title":"Kuby Immunology","author":"Kindt","year":"2006","edition":"6th edn."},{"key":"2022010507305258700_B2","doi-asserted-by":"crossref","first-page":"1703531","DOI":"10.1080\/19420862.2019.1703531","article-title":"Antibodies to watch in 2020","volume":"12","author":"Kaplon","year":"2020","journal-title":"MAbs"},{"key":"2022010507305258700_B3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4161\/19420862.2015.989042","article-title":"The therapeutic monoclonal antibody market","volume":"7","author":"Ecker","year":"2015","journal-title":"MAbs"},{"key":"2022010507305258700_B4","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2217\/nnm.14.178","article-title":"Nanobody-based cancer therapy of solid tumors","volume":"10","author":"Kijanka","year":"2015","journal-title":"Nanomedicine (Lond)"},{"key":"2022010507305258700_B5","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.1016\/j.bbagen.2017.06.014","article-title":"Exploiting sequence and stability information for directing nanobody stability engineering","volume":"1861","author":"Kunz","year":"2017","journal-title":"Biochim. 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