{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T21:54:35Z","timestamp":1778018075142,"version":"3.51.4"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Thirty years have elapsed since the emergence of the classification of carbohydrate-active enzymes in sequence-based families that became the CAZy database over 20 years ago, freely available for browsing and download at\u00a0www.cazy.org. In the era of large scale sequencing and high-throughput Biology, it is important to examine the position of this specialist database that is deeply rooted in human curation. The three primary tasks of the CAZy curators are (i) to maintain and update the family classification of this class of enzymes, (ii) to classify sequences newly released by GenBank and the Protein Data Bank and (iii) to capture and present functional information for each family. The CAZy website is updated once a month. Here we briefly summarize the increase in novel families and the annotations conducted during the last 8 years. We present several important changes that facilitate taxonomic navigation, and allow to download the entirety of the annotations. Most importantly we highlight the considerable amount of work that accompanies the analysis and report of biochemical data from the literature.<\/jats:p>","DOI":"10.1093\/nar\/gkab1045","type":"journal-article","created":{"date-parts":[[2021,10,16]],"date-time":"2021-10-16T02:29:17Z","timestamp":1634351357000},"page":"D571-D577","source":"Crossref","is-referenced-by-count":2125,"title":["The carbohydrate-active enzyme database: functions and literature"],"prefix":"10.1093","volume":"50","author":[{"given":"Elodie","family":"Drula","sequence":"first","affiliation":[{"name":"Aix Marseille Univ, CNRS, UMR7257 AFMB, Marseille, France"},{"name":"INRAE, USC1408 AFMB, Marseille, France"}]},{"given":"Marie-Line","family":"Garron","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, CNRS, UMR7257 AFMB, Marseille, France"},{"name":"INRAE, USC1408 AFMB, Marseille, France"}]},{"given":"Suzan","family":"Dogan","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, CNRS, UMR7257 AFMB, Marseille, France"},{"name":"INRAE, USC1408 AFMB, Marseille, France"}]},{"given":"Vincent","family":"Lombard","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, CNRS, UMR7257 AFMB, Marseille, France"},{"name":"INRAE, USC1408 AFMB, Marseille, France"}]},{"given":"Bernard","family":"Henrissat","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, CNRS, UMR7257 AFMB, Marseille, France"},{"name":"INRAE, USC1408 AFMB, Marseille, France"},{"name":"Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia"},{"name":"Technical University of Denmark, DTU Bioengineering, Kgs Lyngby, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3693-6017","authenticated-orcid":false,"given":"Nicolas","family":"Terrapon","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, CNRS, UMR7257 AFMB, Marseille, France"},{"name":"INRAE, USC1408 AFMB, Marseille, France"}]}],"member":"286","published-online":{"date-parts":[[2021,11,29]]},"reference":[{"key":"2022010507330159700_B1","doi-asserted-by":"crossref","first-page":"D92","DOI":"10.1093\/nar\/gkaa1023","article-title":"GenBank","volume":"49","author":"Sayers","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"2022010507330159700_B2","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"Camacho","year":"2009","journal-title":"BMC Bioinformatics"},{"key":"2022010507330159700_B3","doi-asserted-by":"crossref","first-page":"e1002195","DOI":"10.1371\/journal.pcbi.1002195","article-title":"Accelerated profile HMM searches","volume":"7","author":"Eddy","year":"2011","journal-title":"PLoS Comput. 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Clifton NJ"},{"key":"2022010507330159700_B12","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/nar\/28.1.45","article-title":"The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000","volume":"28","author":"Bairoch","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"2022010507330159700_B13","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1038\/nature21725","article-title":"Complex pectin metabolism by gut bacteria reveals novel catalytic functions","volume":"544","author":"Ndeh","year":"2017","journal-title":"Nature"},{"key":"2022010507330159700_B14","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/s41564-017-0079-1","article-title":"Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides","volume":"3","author":"Luis","year":"2018","journal-title":"Nat. Microbiol."},{"key":"2022010507330159700_B15","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/nature13995","article-title":"Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism","volume":"517","author":"Cuskin","year":"2015","journal-title":"Nature"},{"key":"2022010507330159700_B16","doi-asserted-by":"crossref","first-page":"6063","DOI":"10.1073\/pnas.1815791116","article-title":"Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space","volume":"116","author":"Helbert","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022010507330159700_B17","doi-asserted-by":"crossref","first-page":"17752","DOI":"10.1074\/jbc.RA120.015328","article-title":"A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis","volume":"295","author":"Glasgow","year":"2020","journal-title":"J. Biol. 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